Oxidative damage in DNA

Fig. 9 Long-range oxidative damage in DNA is regulated by the structure of the DNA n-stack. Photoexcited [Rh(phi)2(bpy )]3+ intercalated within B-DNA oxidizes guanine doublets positioned 17 A and 40 A away with approximately equal efficiency. When a bulge is introduced in the DNA oxidation at the distal guanine doublet is inhibited. As shown by the NMR structure, bulges introduce local distortions in the DNA r-stack, although the kinking of the helical actually positions the distal guanine closer to the Rh(III) photooxidant...

Figure 5 Schematic representation of a metaiiated dupiex designed to probe iong-range oxidative damage in DNA. Oxidative damage is found at the S -G of the guanine doubiets.

Odom DT, Barton JK Long-range oxidative damage in DNA/RNA duplexes. Biochemistry 2001, 40(30) 8727—8737. 

The risk of developing colonic cancer is raised in UC, particularly in those with long-standing extensive disease (Lennard-Jones et al., 1990). The mechanism of this increased susceptibility is unknown, although it is tempting to speculate that it is related to inflammation and resulting oxidative damage to DNA. As yet there is little evidence to support this contention. Markowitz et al. (1988) have reported a decrease in constituent and oxidant-induced adenosine diphosphate ribosyl... 

Claycamp, H.G. (1992). Phenol sensitization of DNA to subsequent oxidative damage in 8-hydroxyguanine assays. Carcinogenesis 13, 1289-1292. 

Dizdaroglu, M. (1993). Quantitative determination of oxidative base damage in DNA by stable isotope-dilution mass spectrometry. FEBS Lett. 315, 1-6. 

Astley, SB, Hughes, DA, Wright, AJA, Elliott, RM, and Southon, S, 2004b. DNA damage and susceptibility to oxidative damage in lymphocytes Effects of carotenoids in vitro and in vivo. Br J Nutr 91, 53-61. 

Lynn et al. [71] demonstrated the damaging effect of arsenite on DNA. It has been shown that arsenite at low concentrations increased DNA oxidative damage in vascular smooth muscle cells (VSMCs) that can be a cause of arsenite-induced atherosclerosis. Bruskov et al. [72] found that heat induced the formation of 8-oxoguanine in DNA solution at pH 6.8, which was supposedly mediated by oxygen radicals. 

---