Overpressured layer chromatography flow rate

These limitations have led to the development of forced flow development systems and to the technique of overpressured thin layer chromatography. The special feature of this method is that the adsorbent layer is in a completely sealed unit and the solvent is delivered under pressure at a controlled oniform flow-rate by a pump module as in HPLC. Thus, overpressured TLC (OPTLC) takes place in the absence of a vapour pressure and the migration of the solvent front is free from both evaporation and adsorption effects. As the eluant is delivered under controlled conditions it is possible to optimise the separation conditions by adjusting the flow-rate of the eluant and also to undertake continuous development proeedures. 

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