Overall Procedures and Documentation

FIGURE 8 HPLC chromatograms used for the evaluation of the autosampler. Upper 80 injection of ethylparaben. Lower 80 jiL injection of the mobile phase. 

Sample Data Table for evaluating autosampler sampling accuracy 

FIGURE 9 Gravimetric data table showing the evaluation of autosampler sampling accuracy. 

FIGURE 10 Part of the MS Word template form to document the calibration of the autosampler. A 4-page template was used to document the results obtained for HPLC calibration so any laboratory notebook entries are not needed. Reprinted with permission from Reference 6. 

In summary, this chapter reviews how to develop expedited calibration procedures and acceptance criteria for all the common modules in an HPLC system. 

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