Otsuka Long-Evans Tokushima fatty rat

Effect of CLA Isomers on Hypertension in Otsuka Long-Evans Tokushima Fatty Rats... 

Effects of Short-Term Administration of Conjugated Linoleic Acid on Lipid Metabolism in White and Brown Adipose Tissues of Starved/Refed Otsuka Long-Evans Tokushima Fatty Rats, Food Res. Int. 34 515—520 (2001). 

Troglitazone, a thiazolidine derivative, inhibited the expression of iNOS in adipocytes in 3T3-L1 cells and in Otsuka Long-Evans Tokushima Fatty rats (Dobashi et al. 2000). 

Bajotto, G., Murakami, T., Nagasaki, M., Tamura, T., Tamura, N., Harris, R.A., Shimomura, Y., and Sato, Y., 2004. Downregulation of the skeletal muscle pyruvate dehydrogenase complex in the Otsuka Long-Evans Tokushima Fatty rat both before and after the onset of diabetes mellitus. Life Sciences. 75 2117-2130. 

Kanagawa K, Nakamura H, Murata, et al. Increased gastric acid secretion in cholecystokinin-1 receptor-deficient Otsuka Long-Evans Tokushima fatty rats. Scand J Gastroenterol 2002 37(1) 9-16. 

Kawano, K., Hirashima, T., Mori, S., Saitoh,Y., Kurosumi, M., and Notori, T. (1992) Spontaneous Long-Term Hyperglycemic Rat with Diabetic Complications Otsuka Long-Evans Tokushima Fatty (OLETF) Strain, Diabetes 41,1422-1428. 

Otsuka Long-Evans Tokushima Fatty (OLETF) rats have hyperphagia because they lack receptors for cholecystokinin and become obese, developing hyperlipidemia, diabetes, and hypertension (29-31). We previously reported that CLA, especially Q)trans, 2cis (lOr, 12c) CLA, has anti-obese and hypolipidemic effects in OLETF rats. These effects were attributed to the enhancement of fatty acid 3-oxidation and the suppression of fatty acid synthesis (32,33). In the present study we evaluated the effect of CLA isomers (0.5% supplementation with 5% com oil) on the development of hypertension in OLETF rats. 

Excess carbohydrate intake could be accompanied by a thiamine-defident condition. Therefore, thiamine intervention may impact metabohc abnormalities in Otsuka Long-Evans Tokushima Fatty (OLETF) rats, which were extensively studied as a model of human obesity and metabohc disorders. Based on the assumption that excess glucose influx causes relative thiamine deficiency, we provided thiamine-containing water to the OLETF rats. Thiamine attenuated type 2 DM and metabolic abnormalities in the rat model of human obesity and metabolic disorders. We also investigated the influence of thiamine on PDH activity in the OLETF rats. 

Figure 35.1 Effects of thiamine on body weight and food consumption in OLETF rats. Thiamine decreased body weight (A) despite the equivalent amount of food consumed (B) by the Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Four-week-old male OLETF rats were maintained with water (untreated control group) or water containing 0.2% thiamine (thiamine-treated group). Food consumption relative to body weight, calculated as food consumption (g) divided by 100 g body weight, was measured weekly, and all values are expressed as mean SE to 21 weeks (n=8) and 51 weeks (n = 8) in each group. Modified from Tanaka, Kohda et al. (2010).

Figure 35.3 Genes modified by thiamine intervention in OLETF rats. Thiamine modified transcript expression levels of genes implicated in carbohydrate metabolism, lipid metabolism, vascular physiology and carcinogenesis in the liver of the Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Tanaka and Kohda unpublished data.

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