Other polymer-based SEC columns

Other basic properties of the packings are summarized in Table 8. 

Polyacrylamide has been used as a base material for soft SEC column packings. The new products have high stability to the applied column inlet pressure. According to the supplier, this series of columns can be used even with organic solvents such as dimethyl sulfoxide, tetrahydrofuran, and acetone. The announced properties of the columns are listed in Table 8. 

Column Exclusion Limit (Mw) Column Size (mm) Maximum Press, (kg/cm2) Appiicable Flow Rate (mL/min) Values pH 

SEC column suppliers and dealers provide application data obtained from standard systems, using their columns, in their catalogues, so that one can know the capabilities of their columns. 

The following five figures (Fig.3-7) show typical separations of mixtures containing peptides and proteins on several commercially available SEC columns. 

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