Osteoinductive bone morphogenetic proteins

U. Ripamonti, T. Matsaba, J. Teare et al.. Intrinsic osteoinductivity by smart biomimehc matrices. Abstract in the 4th International Conference on Bone Morphogenetic Proteins, Sacramento, USA, October 17-21, 2002. 

Yuan H, De Braijn JD, Zhang X, Van Blitterswijk CA, De Groot K. Use of an osteoinductive biomaterial as a bone morphogenetic protein carrier. J Mater Sci Mater Med 2001 12 761-6. 

Figure 4.4 Design and fabrication of the osteophilic multilayer architecture. Components of the film (a) Chitosan (75-85% deacetylated chitin) a polycation (b) Poly(acrylic acid) (PAA) (c) A hydrolytically degradable poly(p-amino ester) (Poly2), a polycation and (d) osteoinductive recombinant human bone morphogenetic protein 2 (rhBMP-2) (e) Schematic of the modular electrostatic assembly. Osteoconductive hydroxyapatite (HAP) is complexed with chitosan and incorporated into nanoscale thick films along with poly(acrylic acid) in a bilayer architecture. A hydrolytically degradable poly(P-amino ester)-based multilayer fihn incorporating osteoinductive rhBMP-2 lays atop the osteoconductive layer, (f)...

Recently, a novel macroporous (surface area up to 1.5m cm ), zirconia-based cell carrier biomaterial (Sponceram Figure 10.5), which may be either partially or fully stabilized with Ca, Mg or Y, has been developed that can easily be coated not only with osteoconductive hydroxyapatite but also with the osteoinductive recombinant human bone morphogenetic protein, rhBMP-2. The pore size of this material is about 600 xm, and the porosity may be up to 85% (Roker et al., 2009). Subsequent cell proliferation studies performed in a bioreactor with preosteoblas-tic MC3T3-E1 cells showed that alkaline phosphatase (AP) activity attained a maximum level at day 5 in the presence of rhBMP-2. Likewise, a significant calcification of the extracellular matrix (ECM) occurred in the presence of rhBMP-2,... 

H. Lu, N. Kawazoe, T. Kitajima, Y. Myoken, M. Tomita, A. Umezawa, G. Chen, Y. Ito. Spatial immobiUzation of bone morphogenetic protein-4 in a collagen-PLGA hybrid scaffold for enhanced osteoinductivity. Biomaterials 33,6140-6146,2012. 

Uludag, H., D Augusta, D., Golden, J., Li, J., Timony, G., Riedel, R., and Wozney, J.M. Implantation of recombinant human bone morphogenetic proteins with biomaterial carriers a correlation between protein pharmacokinetics and osteoinduction in the rat ectopic model. /. Biomed. Mater. Res. 50 227-238, 2000. 

H. Saitoh, et ah. Effect of polylactic acid on osteoinduction of demineralized bone preliminary study of the usefulness of polylactic acid as a carrier of bone morphogenetic protein. (0305-182X (Print)). 

---