Organic acids observed in blood and urine using GC-MS

1 Organic acids observed in blood and urine using GC-MS 

Witten et al. (1973) identified adipic and 3-methyladipic acids and also reported the presence in urine, using GC-MS, of aconitic and isocitric acids in addition to citrate. Mamer et al, (1971) reported the occurrence of several hydroxyaliphatic acids in addition to those already identified by other workers, and Mamer and Tjoa have identified 2-ethylhydracrylic acid in urine derived from isoleucine metabolism (Mamer and Tjoa, 1974). Urine from healthy children and adults may contain low amounts of aliphatic dicarboxylic acids of chain length C4-C8 (Lawson et ai, 1976). Pettersen and Stokke (1973) reported a series of 3-methyl-branched C4-C8 dicarboxylic acids in urine from normal subjects, and Lindstedt and co-workers have identified other dicarboxylic acids with cyclopropane rings and acetylenic bonds as well as a series of cis and trans mono-unsaturated aliphatic dicarboxylic acids (Lindstedt et al., 1974,1976 Lindstedt and Steen, 1975). 

Aromatic acids other than hippuric and 4-hydroxyphenylacetic acid and a few of exogenous (dietary) origin are generally observed in urine from normal subjects at very low levels, if at all, since those acids that occur in normal 

All of the identifications described above have been obtained by use of packed columns in the gas chromatograph, from which a normal organic acid 

Considerably less work using GC and GC-MS has been reported on the 

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