Oral Glucose Tolerance Test oGTT in Conscious Rats

Oral Glucose Tolerance Test (oGTT) in Conscious Rats 

On the other hand normal animals are insulin sensitive and further improvement of insulin sensitivity cannot be expected. Therefore, if there are hints from 

OGTT in conscious rats are used to assess the side effect potential of a candidate compound to impair glucose tolerance (Fig. 3). 

Mean values of blood glucose for each time point are calculated and time-course of control and treated groups are compared. Area under the blood glucose curve (AUC) can be calculated and compared for both groups base-line values of blood glucose should be subtracted for the calculation of glucose AUC. 

Many authors used mice instead of rats (Gross et al. 1994, Bailey et al. 1997, Ahren et al. 2000). However, parallel insulin measurements are much more limited in mice than in rats. Also oGTT in insulin resistant animal disease models in mice (e.g. genetic ob/ob, db/db, Ay) are described to assess peripheral insulin resistance (Xiao et al. 2001, Arakawa et al. 2001, Nagakura et al. 2003, Thorkildsen et al. 2003, Minoura et al. 2004). Other routes for glucose administration (e.g. intraperitoneal) are also reported (Xie et al. 2004). 

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