Optical wide-field microscope

Figure30.1 (a) Schematicofthe optical set-upof pulsed laser from the surface of an objective the line-scanning spectromicroscope. (b) Typical scale with a spacing of 10 Xm. (d) Typical image wide-field microscopic image of the offluorescence intensity distribution detected by...

Third, high resolution is required. Wide-field microscope deconvolution systems routinely provide 0.2-ixm XY resolution and approximately 0.4-/x,m resolution along the optical axis. This approaches or equals the theoretical limits for light microscopy, and FISH experiments frequently require this degree of image quality. 

Fig. 1 Schematic drawing of a wide-field single-particle tracking fluorescence microscope equipped. Several lasers are used as excitation source for different fluorophores with fast selection hy an acousto-optical tunable filter (AOTF). The collimated laser light is coupled into the objective such that only the observed area is illuminated. The emission light is separated from the excitation light by a dichroic mirror. In the case of multi-color imaging, the emission light is separated by...

Fig. 8. Wide-field optical microscopy for single-molecule detection. A single lens is introduced in addition to the setup for SCOM of Fig. 5 and the SPAD is exchanged by a fast CCD camera. LI, L2, L3 lenses, S sample, O microscope objective, DH dichroic mirror, F filter.

Fig. 4.11. Left (a) Optical microscope image of an OLED working at a luminance of 100 cd/m2 under water vapor atmosphere. Non-emitting dark spots can be seen clearly, (b) SEM image of the bubbles formed on the aluminum cathode in the dark spot area, (c) Correlation between dark spot growths (taken from the increase in diameter) and total current density [110]. Right (a) Shown here is the random pattern of carbonized areas on the surface of the cathode after operation, shown in wide field, (b) At higher resolution, the structure of one of these areas becomes more apparent, (c) and (d) show nanoscale views of carbonized areas with the extrusion of the polymer through the cathode and the resulting void underneath [111].

The benefit of TCSPC wide-field imaging is that it can be easily adapted to almost any microscope or other optical system. It may also be a solution for samples that preclude, for whatever reason, scanning by a laser spot of high power density. 

What is generally meant by wide-field microscopy is any microscope whereby image formation takes place by the optics without scanning the lens directly forms an image, which can be projected on a camera or observed through the eye piece. Before the development of confocal microscopy and other scanning microscopy modes, this was the only way to perform microscopy. In the biomedical sciences, wide-field fluorescence microscopy is still widely used and offers a number of advantages over confocal and other advanced microscopy modes. 

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