Deconvolution microscope

Figure 11. Analysis of FISH signals using a widefield deconvolution microscope. Seen in this image are xy (top), xz (bottom), and yz (right) projections of deconvolved data. The colocalized FISH signals (yellow) are easily seen in XY and XZ images. Image courtesy. Peter Franklin. Applied Precision Inc, Issaquah, WA, USA.

Fig. 2 Spectral characteristics of an epifluorescence microscope filter cube designed to observe three components simultaneously (DAPI, fluorescein, and Texas Red). Plotted is the transmissivity of the dichroic mirror (lightest line) and the excitation (dark line) and emission (medium line) filters. We routinely use a four-component dichroic mirror in our deconvolution microscope, but for simplicity show the three-component cube here.

Delta Vision deconvolution microscope and Priism software]... 

Figure 6b. Microscopic image of chromosomes improved by blind deconvolution.

Due to the high coherence of electron source in an FEG electron microscope, the information resolution limit of an image can reach the atomic level, much higher than the point resolution. However, the stmcture information, especially those in the high-resolution region, is seriously distorted due to the strong oscillation of the CTF. The distortion can be removed by the image deconvolution technique. 

What is not known, however, is whether image deconvolution can eliminate beam spreading as a contributor to the line scan shape. One would expect that beam spreading from a dense array of sources like the aggregates would obscure features beyond the resolution capabilities of the microscope. In this instance, deconvolution would provide only a modicum of aid in image interpretation. The authors of this entry however believe that beam spreading is a minimal contributor to the image. 

Deconvolution microscopy achieves the same Image-sharpening effect as confocal scanning microscopy but through a different process. In this method, Images from consecutive focal planes of the specimen are collected. A separate focal series of images from a test slide of subresolution size (i.e., 0.2 (jim diameter) bead are also collected. Each bead represents a pinpoint of light that becomes an object blurred by the imperfect optics of the microscope. Deconvolution... 

Why are chemical stains required for visualizing cells and tissues with the basic light microscope What advantage does fluorescent microscopy provide In comparison to the chemical dyes used to stain specimens for light microscopy What advantages do confocal scanning microscopy and deconvolution microscopy provide in comparison to conventional fluorescence microscopy ... 

There are a number of processes that create fast pathways of exchange and effectively short-circuit volume diffusion into a crystal. Thus, the real world may be influenced by crystal defects and dislocations, mineral inclusions, exsolution lamellae, kink bands, microcracks, and other cryptic features (Fig. 12C). Diffusion is always active on a scale that can be modeled (Fig. 12B) and thus a world-view where all minerals are perfectly equilibrated and homogeneous (Fig. 12A) is generally a figment of imagination. In thermometry, these factors all potentially contribute to the compositions that are measured. Major advances have been made in determining when the macroscopic model world accurately predicts the microscopic real world situation. However, more work may be necessary to accurately deconvolute complex cases and tests should always be applied to evaluate thermometry. 

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