Optical enzyme-based sensors spectroscopy

See alsa Distillation. Enzymes Overview Enzyme-Based Electrodes. Food and Nutritional Analysis Antioxidants and Preservatives Alcoholic Beverages. Forensic Sciences Alcohol in Body Fluids Blood Analysis. Headspace Analysis Static Purge and Trap. Infrared Spectroscopy Overview. Optical Spectroscopy Refractometry and Reflectometry. Quality Assurance Quality Control. Sensors Amperometric Oxygen Sensors. 

Inductively Coupled Plasma. Atomic Fluorescence Spectrometry. Atomic Mass Spectrometry Inductively Coupled Plasma. Chemiluminescence Liquid-Phase. Enzymes Enzyme-Based Electrodes. Fluorescence Instrumentation. Ion-Selective Electrodes Overview. Optical Spectroscopy Detection Devices. Sensors Overview. Voltammetry Overview. 

In this part we will describe recent achievements in the development of biosensors based on DNA/RNA aptamers. These biosensors are usually prepared by immobilization of aptamer onto a solid support by various methods using chemisorption (aptamer is modified by thiol group) or by avidin-biotin technology (aptamer is modified by biotin) or by covalent attachment of amino group-labeled aptamer to a surface of self-assembly monolayer of 11-mercaptoundecanoic acid (11-MUA). Apart from the method of aptamer immobilization, the biosensors differ in the signal generation. To date, most extensively studied were the biosensors based on optical methods (fluorescence, SPR) and acoustic sensors based mostly on thickness shear mode (TSM) method. However, recently several investigators reported electrochemical sensors based on enzyme-labeled aptamers, electrochemical indicators and impedance spectroscopy methods of detection. 

This volume presents a cross section of recent advances in the development of novel chemical and biochemical sensors for on-line monitoring and control applications in the environmental, clinical, and bioprocess areas. These chapters illustrate how many of the key challenges for continuous monitoring are being addressed. The methods discussed include optical techniques ranging from near-infrared spectroscopy to lifetime-based phase fluorometry biosensors ranging from optical immu-nosensors to enzyme-electrodes as well as electrochemical, acoustic, and plasmon resonance techniques. 

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