Oligosaccharides in glycoproteins

Kang, S., Cummings, R.D. and McCall, J.W. (1993) Characterization of the A-linked oligosaccharides in glycoproteins synthesised by microfilariae of Dirofilaria immitis. Journal of Parasitology 79, 815-828. 

Nyame, K., Cummings, R.D. and Damian, R.T. (1988) Characterization of the N- and O-linked oligosaccharides in glycoproteins synthesized by Schistosoma mansoni schistosomula. journal of Parasitology 74, 562-572. 

S. A. Carr, M. J. Huddleston, and M. F. Bean, Selective identification and differentiation of N- and O-linked oligosaccharides in glycoproteins by liquid chromatography-mass spectrometry, Protein Sci., 2 (1993) 183-196. 

S., Matsuo, I., and Totani, K., Structural approaches to the study of oligosaccharides in glycoprotein quality control, Curr. Opin. Struct. Biol. 15, 481 89, 2005. 

There are still few ways of achieving the reproducible, partial fragmentation of polysaccharides. Oligosaccharides in glycoproteins and glycolipids are usually sequenced from the outside inward by the use of specific glycosidases and carefully controlled hydrolysis. 

K. Nyame, D.F. Smith, R.T. Damian, R.D. Cummings, Complex-type asparagine-linked oligosaccharides in glycoproteins synthesized by Schistosoma mansoni adult males contain terminal P-linked N -acetylgalactosamine, J Biol Chem, 1989, 264(6), 3235-3243. 

Kurihara, T. Min, J. Z. Toyo oka, T Fukushima, T Inagaki, S. Determination of fluorescence-labeled asparaginyl-oligosaccharide in glycoprotein by reversed-phase ultraperformance Uquid chromatography with electrospray Ionization time-of-flight mass spectrometry. Anal. Chem. 2007, 79, 8694-8698. 

FIGURE 4. Biosynthetic pathway for the formation of core structures of Ser/Thr-linked oligosaccharides in glycoproteins. GTj to GT7 refer to the glycosyltransferases that catalyze the reaction for example, GT, is UDP Gal GalNAca R, pi 3 galactosyltransferase. 

---