Oligosaccharides from glycosidases

Release of Oligosaccharides from Purified Transferrin by N-Glycosidase F Digestion... 

Oligosaccharide syntheses employing enzymatic reactions would in principle greatly benefit from being performed on a polymer support since the support might effectively facilitate isolation of the final product. Presumably, a water-soluble polymeric support will be preferable to any insoluble support since reaction rates could otherwise become too slow. Glycosidases as synthetic enzymes would be the best candidates to study this type of the enzymic approach to oligosaccharide synthesis. 

Singh, S. Scigelova, M. Crout, D. H. G., Glycosidase-catalysed synthesis of oligosaccharides A two-step synthesis of the core trisaccharide of N-hnked glycoproteins using the beta-N-acetylhexosaminidase and the beta-mannosidase from... 

Fig. 4.5.9 HPLC and mass-spectromet-ric analysis of transferrin-linked oligosaccharides. Transferrin is purified from sera of a control and the index CDG-IId patient. Oligosaccharides are released by N-glycosidase F digestion and subsequently analysed by HPLC. Peak fractions of the control and the patient are further investigated by mass spectrometry and are compared to oligosaccharide standards. Values above the HPLC peaks indicate the detected masses...

Both transferases from the Leloir and non-Leloir pathways have been employed for the in vitro synthesis of oligosaccharides and glycoconjugates. Glycosidases have also been exploited for synthesis. The function of glycosidases in vivo is to cleave glycosidic bonds,... 

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