Oligosaccharides enzyme-based synthesis

The challenge for developing methods for synthesizing oligosaccharides, notably for complex oligosaccharides, that can be scaled up to an industrial level is obvious, based on the use of substrates and enzymes that are available at reasonable cost and purity. Strategies for oligosaccharides synthesis include ... 

Fig. 5 Catalytic mechanism of glycogen phosphorylases. The reaction scheme accounts for the reversibility of phosphorolysis of oligosaccharides (R) in the presence of orthophosphate (upper half) and primer-dependent synthesis in the presence of glucose-l-phosphate (lower half). PL enzyme-bound pyridoxal BH-f a general base contributed by the enzyme protein. Reprinted with permission from [109]. Copyright 1990 American Chemical Society...

Based on die highly developed art of oligosaccharide synthesis, orgamc-chemical, enzyme-catalyzed, and combined organic-enzyme-catalyzed syntheses of the Sialyl-Lewis structure and related oligosaccharides have been devised. Selected examples of these syntheses are discussed in Section IV, some of them including recently developed procedures of glycoside formation. 

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