Oligosaccharides chemical synthesis

Chemical synthesis of oligosaccharides related to arabinogalactan-proteins (agps) J.F. Valdor and W.Mackie... 

The chemical synthesis of oligosaccharides is also more complicated than the synthesis of other biopolymers, because the construction of each individual oligosaccharide poses a new challenge, requiring a knowledge of methods, together with experience and experimental skill. Thus, there are no universal methods available neither for biological in vivo nor for chemical in vitro syntheses. 

Oligosaccharides and glycoconjugates in living cells often exist as closely related mixtures. Their isolation from natural sources in homogeneous form is therefore very difficult, involving tedious purification and difficult characterization. This sequence of steps tends to result in low yields. This difficult situation presents chemical synthesis with a major opportunity to positively affect progress in the biochemical understanding of the processes described above.4... 

Thorough, advanced treatment of the chemistry and biology of cell surface carbohydrates. Good chapters on lectins, carbohydrate recognition in cell-cell interactions, and chemical synthesis of oligosaccharides. 

A review of applications of chemical synthesis of carbohydrates to an understanding of the biological roles of oligosaccharides. 

H. Paulsen, Progress in the chemical synthesis of complex oligosaccharides, Angew. Client Int. Ed. Engl. 21 155 (1982). 

R. R. Schmidt, Chemical synthesis of silylated glycoconjugates, in Synthetic Oligosaccharides-Indispensible Probes for the Life Sciences, P. Kovac, ed., American Chemical Society, Washington DC, 1994, p. 276. 

Despite the great deal of progress made in the chemical synthesis of oligosaccharides, there is still room for innovation and improvement over existing methods. The need for rapid and efficient construction of oligosaccharides has dramatically increased owing to the recent advances in glycobiology and related research fields [21],... 

The third theme is concerned with the chemical synthesis of O- and Af-glycosy compounds, and of oligosaccharides, and is the subject of ten chapters. The most widely used methods for glycoside synthesis are discussed, together with the inclusion of concept tually new approaches to anmneric activation and glycoside synthesis. 

In order to try to overcome some of the problems associated with chemical synthesis of oligosaccharides containing N-acetylneuraminic acid, Sabesan and Paulson [277] have used a combination of chemical and enzymatic methods using purified sialyl-transferases in the presence of CMP-iV-acetylneuraminic acid and synthetic acceptor molecules to give sialyl derivatives of oligosaccharides which were characterised by NMR. Thus, methyl P-D-galactopyranoside, methyl P-D-lactoside and iV-acetyl-... 

We have developed an automated solid-phase approach for the chemical synthesis of oligosaccharides. Using an acceptor-bound solid-phase... 

Khan SH, Hindsgaul O (1994) Chemical synthesis of oligosaccharides. In Fukuda M, Hindsgaul O (eds) Frontiers in Molecular Biology, Molecular Glycobiology. IRL Press, Oxford, p 206... 

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