Oligosaccharide synthesis sequence analysis

While the path to enhancing automated synthesis is clear, a number of practical challenges remains. Given the recent success of synthetic methods, other bottlenecks have emerged. Notably, the purification of both fully protected and deprotected oligosaccharides is a laborious process. Improvements in currently available techniques are needed to allow for the expedient purification of synthetic material. More efficient methods for the analysis and sequencing of carbohydrates are of major importance. 

A review containing 32 references has been published on sequencing methods applicable in the structural analysis of oligosaccharides. A further review by Whitesides and coworkers has dealt with the enzymic in vitro synthesis of mono-, oligo- and poly-saccharides, and the effects of several parameters on the yields and selectivities of glycosidase-catalysed formation of oligosaccharide glycosides have been discussed. ... 

A review has appeared on the synthesis of oligosaccharides, glycopeptldes and complex oligosaccharides of glycoproteins and on the conformational analysis of the oligosaccharide sequences. ... 

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