Oligoribonucleotides

The capped leader sequence of the brome mosaic virus mRNA, an oligonucleotide 5 triphosphate, has been synthesized analogously by reaction of an oligoribonucleotide 5 -phosphoric imidazolide. 951... 

Such compounds have been used in the solid-phase synthesis of oligoribonucleotides. In the synthesis of (Up)8U, for example, the coupling reaction gave a yield of 96%.[105]... 

In ref. [178] other tri(azolyl)phosphines are used for the synthesis of uridine-, adenosine-, and cytidine-oligoribonucleotides according to this method, with azolyl groups... 

Pace, B., and Pace, N.R. (1980) The chromatography of RNA and oligoribonucleotides on boronate-substituted agarose and polyacrylamide. Anal. Biochem. 107, 128-135. 

An interest in ester migration in the monoacyl derivatives of the vicinal-diol grouping in ribofuranosides stemmed from a desire to establish the position of attachment of the aminoacyl group to the terminal adenosine residue of aminoacyl-(t-RNA),569-571 and also from the necessity of producing suitably protected intermediates for oligoribonucleotide syntheses. That a facile equilibrium can exist is shown by the fact that, on being kept, an ethanol solution of... 

Hager and Szostak used an RNA library in which each member was capped by an adenosine-5 -5 -pyrophosphate group at the 5 -end to isolate ribozymes that catalyze the ligation of an oligoribonucleotide to this activated group. This reaction results in the formation of a 3 -5 -ligation and the release of AMP [82]. 

In common with the digestion of other macromolecules, nucleic acids are hydrolysed in a stepwise manner, by pancreatic nuclease (diesterase enzymes) which hydrolyse the bonds between two adjacent phosphate groups in RNA and DNA. The resultant oligoribonucleotides and oligode-oxy ribonucleotides are hydrolysed to form nucleoside monophosphates, which lose their phosphate to form nucleosides, by the action of pancreatic phosphatase. In brief, the process is ... 

The on state of the eukaryotic cytoplasmic A-site has been recently solved by X-ray analysis of model oligoribonucleotide containing two A-sites. Its overall... 

Uhlenbeck, O.C. (1987) A small catalytic oligoribonucleotide. Nature, 328.596-600. Zaug, A.J., Michael, D.B. and Cech. T.R. (1986) The Tetrahymena ribozyme acts like an RNA restriction endonuclease. Nature, 324, 429 33. 

The synthesis sketched in Figure 16.12 can be used to prepare oligodeoxynucleo-tides of up to about 150 bases, and examples of the preparation of cyclic oligodeoxy-nucleotides [122] and oligoribonucleotides [131] using this technique have also been... 

Oligoribonucleotides are more difficult to handle than oligodeoxyribonucleotides, mainly because of their sensitivity towards nucleases. For the synthesis of oligoribonucleotides on insoluble supports, protection of the 2 -hydroxyl group is required, which must be stable towards oxidation with iodine and the acidic conditions of DMT depro-... 

A. Vasella Oligoribonucleotide foldamers with a novel architecture... 

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