Branched oligoribonucleotides

Damha, M. J., Ganeshan, K., Hudson, R. H., and Zabarylo, S. V. (1992). Sobd-phase synthesis of branched oligoribonucleotides related to messenger RNA spbcing intermediates. Nucleic Adds Res. 20, 6565-6573. 

As RNA synthesis has become routine, the synthesis of more complex oligomers has been investigated. One particular area of interest in RNA synthesis is the formation of cyclic and lariat RNA. The use of a protected universal building block containing the selectively activated 2 -H-phosphonate and 3 -chlorophenylphosphate has been used to prepare branched oligoribonucleotides and lariat structures."" The synthesis of small to medium cyclic RNA can be carried out, providing that the linear precursor attached to the support has a... 

Fig 3 Reversed-phase HPLC analysis of the mixtures generated by treatment of branched oligoribonucleotide IV (Table 3) with SVPDE/AP and nuclease PI/ AP. Inosine results from the deamination of adenosine by adenosine deaminase, which is a contaminant in the enzyme preparations. The peak appearing at ca. 1 min is a nonnucleotidic material HPLC conditions Section 3.4.6... 

The chemical synthesis of oligoribonucleotides containing vicinal 2, 5 - and 3, 5 -phosphodiester links, which are found at the branching site of lariat mRNA during mRNA splicing, has been reviewed.267 The synthesis of a substantial number of triribonucleoside diphosphates (184) has been reported analogues of (184 ... 

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