Oligonucleotide solid-phase

To enable a covalent labeling of target molecules, the dyes are functionalized with reactive groups. These are either phosphoramidites for the labeling of synthetic oligonucleotides (solid-phase synthesis), or uridine-5 -triphosphates for enzymatic coupling in PCR (random primed labeling... 

Operational equations from our FA model have been used to estimate FA fluxes, turnover rates, and half-lives within brain phospholipids in vivo, after values for the dilution factor X of the acyl-CoA pool were experimentally determined. This can be accomplished by a rapid method involving oligonucleotide solid-phase extraction (Deutsch et al., 1997). Low experimental values for X in the awake rat, between 0.02 and 0.04, are consistent with very short half-lives resulting from de-esterificafion-re-esteri-fication of FAs within some phospholipids (minutes to hours), very rapid turnover rates and high ATP consumption (Purdon Rapoport, 1998). 

Hurley, D. J., Tor, Y. (1998). Metal-containing oligonucleotides Solid-phase synthesis and luminescence properties, J. Am. Chem. Soc., 120 2194. 

Taylor JS, Brockie IR, O Day CL (1987) A building block for the sequence-specific introduction of ds-syn thymine dimers into oligonucleotides, solid-phase synthesis of TpT[c,s]pTpT. J Am Chem Soc 109 6735... 

The protected nucleoside-3-phosphoramidite monomer units such as 671 are used in the solid-phase oligonucleotide synthesis. In the 60mer synthesis, 104 allylic protective groups are removed in almost 100% overall yield by the single Pd-catalyze reaction with formic acid and BuNH2[432], N,(9-protection of uridine derivatives was carried out under pha.se-transfer conditions[433]. 

Synthetic organic polymers, which are used as polymeric supports for chromatography, as catalysts, as solid-phase supports for peptide and oligonucleotide synthesis, and for diagnosis, are based mainly on polystyrene, polystyrene-divinylbenzene, polyacrylamide, polymethacrylates, and polyvinyl alcohols. A conventional suspension of polymerization is usually used to produce these organic polymeric supports, especially in large-scale industrial production. 

One widely used method involving protected compounds is solid-phase synthesis (polymer-supponed reagents). This method has the advantage of requiring only a simple workup by filtration such as in automated syntheses, especially of polypeptides, oligonucleotides, and oligosaccharides. 

Solid-phase chemistry is an efficient synthetic tool that, compared with solution-phase chemistry, simplifies the work-up of the reaction, allows the process to be driven to completion by using excess of reagents, and can be automatized [2a]. In recent years, many studies have been devoted to developing both surface-mediated and resin-supported synthesis. Today the solid-phase approach is not limited to peptides and oligonucleotides but is also used to synthesize molecules of lower molecular weight. 

Synthetic oligonucleotides are very important tools in the study and manipulation of DNA, including such techniques as site-directed mutagenesis and DNA amplification by the polymerase chain reaction. The techniques for chemical synthesis of oligonucleotides are highly developed. Very efficient automated methodologies based on solid phase synthesis are used extensively in fields that depend on the availability of defined DNA sequences.52... 

Silica or porous glass is usually used as the solid phase in oligonucleotide synthesis. The support is functionalized through an amino group attached to the silica surface. There is a secondary linkage through a succinate ester to the terminal 3 -OH group. 

Over the past decade the techniques of combinatorial synthesis have received much attention. Solid phase synthesis of polypeptides and oligonucleotides are especially adaptable to combinatorial synthesis, but the method is not limited to these fields. The goal of combinatorial synthesis is to prepare a large number of related... 

---