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Oligonucleotides biological synthesis

S. Agrawal (ed.). Protocols for Oligonucleotide Conjugates, Synthesis and Analytical Techniques, Methods in Molecular Biology, 26, Humana Press, Inc., Totowa, New Jersey, 1994. [Pg.108]

Oligonucleotides—Synthesis. 2. Oligonucleotides—Derivatives-Synthesis. 1. Agrawal, Sudhir. II. Series Methods in molecular biology (Totowa, NJ) 20. [Pg.507]

Scheme 1. The biological synthesis of oligonucleotides proceeds via two steps, the first being a reversible ligation of the nucleotide triphosphate, and the second an irreversible hydrolysis of pyrophosphate. (Reproduced with permission from reference 41. Copyright 2007 Verlag Helvetica Chimica Acta.)... Scheme 1. The biological synthesis of oligonucleotides proceeds via two steps, the first being a reversible ligation of the nucleotide triphosphate, and the second an irreversible hydrolysis of pyrophosphate. (Reproduced with permission from reference 41. Copyright 2007 Verlag Helvetica Chimica Acta.)...
The minimal cell, as the simplest system which has all the required properties of life (metabolism, self-reproduction and the ability to evolve), is presently studied as part of a new research discipline synthetic biology. This includes subjects such as synthesis in branches of biological systems, for example, of new RNA species, new peptides and new nucleic acid analogues, as well as the synthesis of peptide nucleic acids. One example is the work of M. R. Ghadiri and G. von Kiedrowski on self-replication of oligonucleotides and oligopeptides (Luisi, 2006b). [Pg.264]

In contrast to the biopharmaceuticals discussed thus far (recombinant proteins and gene therapy products), antisense oligonucleotides are manufactured by direct chemical synthesis. Organic synthetic pathways have been developed, optimized and commercialized for some time, as oligonucleotides are widely used reagents in molecular biology. They are required as primers, probes and for the purposes of site-directed mutagenesis. [Pg.451]


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