Oligonucleotides assays

Drolet DW, Moon-McDermott L, Romig TS, An enzyme-linked oligonucleotide assay, Nat. Biotech., 14 1021-1025, 1996. 

DNA Isolation Techniques for Allele-Specific Oligonucleotide Assay or Gel Denaturing and Polymerase Chain Reaction... 

Load 5 pL of the samples and run the gel at 120 W until the bromophenol blue reaches the 3/4 of the plates for the oligonucleotides assays, or until the bromophenol blue reaches the bottom of the gel for the pBluescript SK(-) fragment assays. If necessary, decrease electric power in order not to exceed 50°C during the electrophoresis (to avoid cracking of the glass plates). 

Kreklau EL, Limp-Foster M, Liu N, Xu Y, Kelley MR, Erickson LC (2001) A novel fluorometric oligonucleotide assay to measure O(6)-methylguanme DNA methyltransferase, methylpurine DNA glycosylase, 8-oxoguanine DNA glycosylase and abasic endonuclease activities DNA repair status in human breast carcinoma cells overexpressing methylpurine DNA glycosylase. Nucleic Acids Res 29 2558-66... 

The second experiment was carried out with the same RNA isolated from mycelia growing on presence of glucose and apple pectin. cDNAs were obtained from those RNA by reverse trancription and used as template for PCR assays. Specific oligonucleotide primers for PG gene were used in the amplification reaction. The Fig-5 shows the results of this amplification experiment. 

Primers The primers are short (15-30) oligonucleotide sequences designed to base pair or anneal to complementary sequences that flank the DNA target sequence to be amplified. The primers are added at 0.1-1 qM in the assay. 

Zhang W (2006) Microwave-Enhanced High-Speed Fluorous Synthesis. 266 145-166 Zhang X-E, Deng J-Y (2005) Detection of Mutations in Rifampin-Resistant Mycobacterium Tuberculosis by Short Oligonucleotide Ligation Assay on DNA Chips (SOLAC). 261 169-190... 

Cyanine dyes also are used as labels for oligonucleotide probes. Unlike the hydrophilic cyanine dyes valuable for protein labeling, the use of dye-phosphoramidite compounds to synthesize DNA or RNA probes typically requires the use of more hydrophobic dye structures to make them compatible with the solvents and reactions of oligonucleotide synthesis. Thus, indol cyanines containing few or no sulfonates are used in these applications to label oligos for applications such as array detection, hybridization assays, and RT-PCR. 

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