Enzyme-linked oligonucleotide sorbent assay

Hepatitis B surface-antigen (HBsAg) 1995 C Factor 100 (IPCR 0.5 pg RIA 50 pg) Maia et al. [30], sandwich IPCR with subsequent PCR-enzyme linked oligonucleotide sorbent assay (see also Fig. 6 and Section 2.2.2)... 

In the multiplex-IPCR assays carried out by Joerger and Hendrickson, DNA of different lengths was used for separation of the PCR amplificates (see Fig. 4). Results of their experiments are discussed below in Section 3.5. The ability to discriminate between different DNA markers by length is nevertheless limited by the separation capabilities of the gel. For a large number of different DNA probes, a sequence-specific detection carried out, for example, by PCR-enzyme-linked oligonucleotide sorbent assay (ELOSA Section 2.2.2) should be preferable. 

Immunoassays for nucleic acids usually employ a solid phase on which the analyte is immobilized either before or after hybridization. Two general configurations, immunocapture or hybridization capture (enzyme-linked oligonucleotide-sorbent assay or ELOSA), are possible (Figure 1). Careful design and optimization of each component can yield a high-performance assay. Typical components for a microwell PCR immunoassay are provided in Table 1. 

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