Oligonucleotide ligation assays OLA

Another example is Oligonucleotide Ligation Assay (OLA), which utilizes an enzyme lig-ase. The ligase connects oligonucleotide strands annealed side by side, that is with no gap between the two strands. If there is even a one-base gap between them, no (or only suppressed)... 

Baron H, Fung S, Aydin A, et al. (1996). Oligonucleotide ligation assay (OLA) for diagnosis of familial hypercholesterolemia. Nat. Biotechnol. 14 1279-1282. 

DASH, dynamic allele-specific hybridization AS-PCR, allele-specific polymerase chain reaction AS-PE, allele-specific primer extension APEX, arrayed primer extension FP-TDI, fluorescence polarization template directed dye terminator incorporation MALDI-TOF-MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry OLA, oligonucleotide ligation assay RCA, ... 

Another technique, now widely used to construct tests for conditions like CF where there are a large number of possible mutations, all resulting in essentially the same phenotype, is the PCR-oligonucleotide ligation assay (PCR/OLA). In this technique multiplex PCR reactions are followed by a multiplex oligonucleotide ligation reaction which is made up of three probes for each biallelic site to be looked at. One probe is common and is... 

---