Oligonucleotide hydrazide-modified

Conjugation of SFB-Modified Alkaline Phosphatase to Bis-Hydrazide-Modified Oligonucleotides... 

Figure 27.17 SFB may be used to create aldehyde groups on enzyme molecules for subsequent conjugation to a 5 -bis-hydrazide-modified oligonucleotide, forming hydrazone bonds.

Figure 1.112 Phosphate groups can be modified with adipic acid dihydrazide in the presence of a carbodi-imide to produce hydrazide derivatives. This is a common modification route for the 5 -phosphate group of oligonucleotides.

Biotin-hydrazide has been used to biotinylate antibodies at their oxidized carbohydrate residues (O Shanessy et al., 1984, 1987 O Shanessy and Quarles, 1985 Hoffman and O Shannessy, 1988), to modify the low-density lipoprotein (LDL) receptor (Wade et al., 1985), to biotinylate nerve growth factor (NGF) (Rosenberg et al., 1986), and to modify cytosine groups in oligonucleotides to produce probes suitable for hybridization assays (Reisfeld et al., 1987) (Chapter 27, Section 2.3). 

DNA-conjugated polymers were prepared from polyallylamine or polyacrylic acid (PAA) modified with ssDNA as a probe, and thioctic acid (TA) or 3-(pyridyldithio)propionyl hydrazide (PDPH) for self-assembled immobilization. The oligonucleotides sequences of the probes and of the fully matched and unmatched DNAs were S -TCC-TCT-TCA-TCC-TGC-TGC-TAT-GCC-TCA-TCT-T-R, 5 -AGA-TGA-GGC-ATA-GCA-GCA-GGA-TGA-AGA-GGA-3, ... 

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