Alkaline phosphatase modified

Alkaline Phosphatase Conjugation to Cystamine-Modified DNA Using Amine- and Sulfhydryl-Reactive Heterobifunctional Crosslinkers... 

Alkaline Phosphatase Conjugation to Diamine-Modified DNA Using DSS... 

Conjugation of SFB-Modified Alkaline Phosphatase to Bis-Hydrazide-Modified Oligonucleotides... 

In this method the single-stranded DNA fragment to be sequenced is end-labelled by treatment with alkaline phosphatase to remove the 5 phosphate, followed by reaction with 32P-labelled ATP in the presence of polynucleotide kinase, which attaches 32P to the 5 terminal. The labelled DNA fragment is then divided into four aliquots, each of which is treated with a reagent which modifies a specific base as follows. 

Studies of acid and alkaline phosphatase activities in bone have been used previously to investigate the action of various substances such as parathormone223, 224, calcitonin225, diphosphonates226 and vitamin A227. Calvarial acid and alkaline phosphatase activities are modified after in vivo injections of vitamin D3 metabolites to rats fed a vitamin D-deficient, low-calcium (0.02 %)diet. Changes in these activities are observed after administration of small doses, i. e. 

At room temperature alkaline phosphatases are generally stable in neutral or mildly alkaline solution but are sensitive to inactivation by acid. Unfortunately, most stability data refer to impure preparations and some of the following statements may need modifying when further information is available. Scutt and Moss investigated the denaturation of human liver and intestinal enzymes at pH 2.1 and 0° (92). The liver enzyme was significantly more labile, and both enzymes could be par-... 

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