Ogston effect

Ratio of areas under a- and jS-peaks [not corrected for Johnston-Ogston effect (1946)]. 

Lewis and Piez (1961). Ratio is corrected for the Johnston-Ogston effect. 

S( is used to denote flotation rates in a medium of 1.745 molal NaCl (pje = 1.0630 gm/ml), whereas such flotation rates corrected for F versus C effects and Johnston-Ogston effects are designated by Sj°. Flotation rates at other densities are designated by the symbol F with a subscript indicating the density. The symbol F appearing alone indicates flotation rates at an arbitrary density. 

Fig. 4a and b. A normal male low-density pattern (film 393) reconstructed from computer-calculated concentrations in the cell for 29 standard intervals at each stage of the program calculations curve 1 represents unoorrected concentrations (corresponding to the tracing of the original schlieren pattern) curve 2 shows the rephasing due to the F versus C correction curves 3 and 4, respectively, demonstrate the additional effects of the correction to standard conditions of temperature and density and the correction for the Johnston-Ogston effect. 

These notations are carried through the calculations, with prime marks indicating the last level of correction that has been applied single prime refers to values corrected for F rate versus concentration dependency, double prime to values following the correction to standard conditions of flotation, and triple prime to values corrected for the Johnston-Ogston effect. 

Although the Johnston-Ogston effect has been rigorously treated for a two-component system (if), there appear to be a number of problems associated with applying this correction to a continuous distribution of macromolecules. We have attempted to estimate its contribution as a series of n(n-l)/2 concentration differences, beginning our ealculation at the base of cell. From the m" the program calculates the boundary F-rate of each component and initially sets c" equal to c". Then... 

Fig. 7. Comparison profiles of 16 males and 16 females for both low- and high-density lipoprotein spectra, constructed from mean base-of-cell corrected concentrations for the standard intervals of each run type. Averaged concentrations have been F versus C corrected, corrected to standard conditions of temperature and density, and corrected for the Johnston-Ogston effect preceding the base-of-cell correction.

The second factor is the Johnston-Ogston effect, which is related to the multicomponent system. If a solution contains the fast and slow components and... 

The concept underlying this specific behavior is called the Ogston effect (61). A. G. Ogston suggested in 1948 that in order for an enzyme to be capable of asymmetric attack upon a symmetric substrate, it must bind the substrate to at least three points. Only then stereoselective recognition or synthesis is possible. 

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