Johnston-Ogston effect

Ratio of areas under a- and jS-peaks [not corrected for Johnston-Ogston effect (1946)]. 

Lewis and Piez (1961). Ratio is corrected for the Johnston-Ogston effect. 

S( is used to denote flotation rates in a medium of 1.745 molal NaCl (pje = 1.0630 gm/ml), whereas such flotation rates corrected for F versus C effects and Johnston-Ogston effects are designated by Sj°. Flotation rates at other densities are designated by the symbol F with a subscript indicating the density. The symbol F appearing alone indicates flotation rates at an arbitrary density. 

Fig. 4a and b. A normal male low-density pattern (film 393) reconstructed from computer-calculated concentrations in the cell for 29 standard intervals at each stage of the program calculations curve 1 represents unoorrected concentrations (corresponding to the tracing of the original schlieren pattern) curve 2 shows the rephasing due to the F versus C correction curves 3 and 4, respectively, demonstrate the additional effects of the correction to standard conditions of temperature and density and the correction for the Johnston-Ogston effect. 

These notations are carried through the calculations, with prime marks indicating the last level of correction that has been applied single prime refers to values corrected for F rate versus concentration dependency, double prime to values following the correction to standard conditions of flotation, and triple prime to values corrected for the Johnston-Ogston effect. 

Although the Johnston-Ogston effect has been rigorously treated for a two-component system (if), there appear to be a number of problems associated with applying this correction to a continuous distribution of macromolecules. We have attempted to estimate its contribution as a series of n(n-l)/2 concentration differences, beginning our ealculation at the base of cell. From the m" the program calculates the boundary F-rate of each component and initially sets c" equal to c". Then... 

Fig. 7. Comparison profiles of 16 males and 16 females for both low- and high-density lipoprotein spectra, constructed from mean base-of-cell corrected concentrations for the standard intervals of each run type. Averaged concentrations have been F versus C corrected, corrected to standard conditions of temperature and density, and corrected for the Johnston-Ogston effect preceding the base-of-cell correction.

The second factor is the Johnston-Ogston effect, which is related to the multicomponent system. If a solution contains the fast and slow components and... 

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