Ocean Spray Cranberries, Inc

Ocean Spray Cranberries, Inc., One Ocean Spray Drive, Lakeville-Middleboro, MA 02349... 

Financial support was provided through USDA-CSREES-NRI Award 2002-353030-12297. 90MX spray-dried cranberry juice powder was provided by Ocean Spray Cranberries, Inc. 

Cranberry seeds were obtained from Ocean Spray Cranberries, Inc. (Lakeville-Middleboro, MA) and stored in polyethylene containers at 10°C. The seeds were freshly ground prior to each extraction. HPLC grade methanol, water, and toluene were obtained from Burdick Jackson Laboratories, Inc. (Muskegon, MI), acetyl chloride was obtained from Sigma-Aldrich Chemical Co. (Milwaukee, WI), and sodium carbonate and Ottawa Sand Standard (20-30 mesh) were obtained from Fischer Scientific (Houston, TX). The internal standard, Cn triglyceride (triundecanoin), was obtained from Nu-Check Prep, Inc. (Elysian, MN). CO2 (SFC/SFE grade) with helium headspace was supplied by Air Products and Chemicals, Inc. (Allentown, PA). 

The DPPH assay (5) was used to evaluate the free-radical-scavenging capacity of extracts prepared by the methods outlined above, whole berry extract and commercial cranberry juice cocktail (Ocean Spray Inc.). Activity was compared to that of a standard antioxidant (Vitamin E, Aldrich Chemical Co.) measured using the same methods. Varying concentrations of cranberry extracts were mixed with a 60 pM solution of DPPH in methanol. Quenching of the violet DPPH radical was observed as a decrease in absorbance at 515 nm over one hour. EC50 values are measured as the sample concentration required to decrease DPPH absorption by 50%. Results are shown in Table I. The DPPH assay was also used to evaluate the extracts of peel, solids and juice EC50 values are reported in Table V. 

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