Nystatin determination

Galego and Arroyo [14] described a simultaneous spectrophotometric determination of OTC, hydrocortisone, and nystatin in the pharmaceutical preparations by using ratio spectrum-zero crossing derivate method. The calculation was performed by using multivariate methods such as partial least squares (PLS)-l, PLS-2, and principal component regression (PCR). This method can be used to resolve accurately overlapped absorption spectra of those mixtures. 

United States Pharmacopoeia 28 [1] describes a microbiological method under antibiotics-microbial assays for the analysis of OTC and nystatin capsules, OTC and nystatin for oral suspension, OTC HC1 and hydrocortisone ointment, and OTC HC1 and polymyxin B sulfate ointment. The methods are relative rather than absolute, which are based on the determination of the level of oxytetracycline by a microbiological response to a series of standard oxytetracycline concentrations by a... 

Pharmaceutical powder is a mixture of finely divided drugs and/or chemicals in dry form. They are dispensed as bulk powders or divided powders. When the prescription is received for powders, first determine whether it is based upon one unit or upon a bulk formula to be subdivided into individual units. Bulk powders are provided as multiple doses in a container and the patient measures the dose as instructed at the time of administration. Some examples of bulk powders include Tolnaftate Powder USP and Nystatin Topical Powder USP as antifungals, and Desitin Powder for diaper rash. Divided powders are meant to be provided as single dose units in individually wrapped powder papers. Such single dose packets are stacked in a powder box, and the label... 

Maertlbauer et al. [34] developed a highly specific and sensitive enzyme immuno assay for the quantitative determination of natamycin in cheese rind. Rabbits were immunized with a natamycin-HSA conjugate to produce a specific antiserum. A labelled ligand was produced by coupling natamycin to horseradish peroxidase. The range of this ELISA test was between 0.2 and 2 ng per ml of sample solution. This allowed the determination of natamycin in cheese rind down to concentrations of 5 ng/dm2 or 0.1 mg/kg. The recovery in a range of 1 to 80 mg/kg was 76 to 84%. The cross-reactivity with amphotericin B and nystatin was <0.001%. 

Nystatin is an amphtoteric compound widi... a carboxyl and an amino function. Ray-Johnson determined die ionization constants of nystatin in a mixture of N,N-dimethylformamide / water (50 50) by direct titration and— following die general procedure of Albert and Serjeant—calculated the following pJC values from the respective equilibrium constants pKi (proton gained)... 

Valenti determined the ionization constants. .. of nystatin in a. .. solvent system composed of methanol, 2-medioxyethanol and water by potentiometric titration and established the following apparent pJCa values. .. pJCi = 5.72 p 2 = 8.64.. .. The isoelectric point for nystatin in diis system, calculated from die average of pKi and pJC2/ was found to be at pH 7.18.. .. There is, as yet, no experimental evidence to establish whether nystatin exists at the isoelectric point as a zwitterion or as an unionized molecule. Resolution of diis question requires the examination of singly charged derivation of the antibiotic, such as an ester and / or suitable salt. 

Scheuch, E. GieOmann, T. Siegmund, W. Quantitative determination of nystatin in human plasma using LC-MS after inhalative administration in healthy subjects. J. Chromatogr. B, 2006, 844, 84-88. 

C N.m.r. has been used to distinguish between 7- and 9-purine substitution in ribofuranosyl nucleosides useful signals are those of C-1 which differ for the two types and those of the carbon atoms of the purine moiety which are characteristic for the two types of substitution. The anomeric configuration of mycosamine in the polyene antifungal antibiotics lucensomycin and nystatin has been determined by C n.m.r. the former contains an a-mycosaminyl residue while the latter is a j8-mycosaminide. ... 

Groll, A.H. Mickiene, D. Werner, K. Piscitelli, S.C. Walsh, T.J. High-performance liquid chromatographic determination of Uposomal nystatin in plasma and tissues for pharmacokinetic and tissue distribution studies, J.Chromatogr.B, 1999, 735, 51-62. 

Mascini M., Memoli A. and Olana F. (1987) Electrochemical biosensors for determination of nystatin activity. Anal. Chim. Acta. 200, 237-244. 

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