Nucleus Transmission electron microscopy

Interestingly, the cellular distribution was analyzed by energy-filtered transmission electron microscopy and electron tomography, demonstrating the presence of free fullerene in the cytoplasm, or associated with nuclear membrane, plasma membrane, lysosomes and, rather surprisingly, with the nucleus (Porter et al., 2006, 2007). 

Porter AE, Gass M, Muller K, Skepper JN, Midgley P, Welland M (2007) Visualizing the uptake of C60 to the cytoplasm and nucleus of human monocyte-derived macrophage cells using energy-filtered transmission electron microscopy and electron tomography. Environ. Sci. Technol. 41 3012-3017. 

Fig. 4 Transmission electron microscopy of a longitudinal section of the posterior end of a Cryptosporidium parvum sporozoite showing immunogold localization of pyruvate NADP+ oxidoreductase (CpPNO). The mitochondrion-like organelle ( ) is posterior to the nucleus, and lies between the nucleus and the CB. It is labeled by mitochondrion-specific 15-nm gold anti- particles. Small -nm gold goat anti-CpPFO particles (arrows) show the localization of CpPNO. There are no 6-nm particles localized within the mitochondrion-like organelle (reprinted from Fig. 12 of Ctrnacta et al. 2006 with permission of the publishers)...

Within vacuolar forms, MLOs are usually situated within the cytoplasmic rim, near the nucleus, and the configuration of a nuclei flanked on each side by one or more MLO is often observed in transmission electron microscopy ( ) sections (Fig. 2) (Zierdt 1986 Dunn et al. 1989 Stenzel et al. 1994 Stenzel and Boreham 1996). However, there have been reports of cytoplasmic invagination resulting in organelles, including MLOs, being deposited within the... 

Transmission electron microscopy (TEM) images showed that GNPs conjugated with Tat peptide (nuclear localization sequence) were mainly located in the nucleus of fibroblast and also revealed no appreciable cytotoxic effects [22]. In contrast, Tkachenko et al. functionalized GNPs with four different nuclear localization peptides ending with a Cys residue (which contains a free thiol) to evaluate the cellular trajectories of peptide gold complexes. Functionalized GNPs were... 

Fig. 4.1. Lactic acid bacteria isolated in wine under a scanning electron microscope (Departement de Microscopie Electronique, University of Bordeaux I), (a) Photograph of Lactobacillus plantarum cells (transmission, Lonvaud, 1975) c = cytoplasm pm = plasma membrane cw = cell wall s = septum m = mesosome n = nucleus, (b) Photograph of I uconostoc oenos Oenococcus oeni) (scanning electron microscope).

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