Electron tomography

Frank, J., Electron Tomography—Three-dimensional Imaging with the Transmission Electron Microscope, Plenum Press, New York, 1992. 

Kurner, J., Frangakis, A.S. and Baumeister, W. (2005). Cryo-electron tomography reveals the... 

Perkins, G.A., Reuken, C., Martone, M.E., Young, S.J. and Frey, T. (1997). Electron tomography of neuronal mitochondria three dimensional structure and organisation of crystae and membrane contacts. J. Struct. Biol., 119, 260-272... 

He, W., Cowin, P. and Stokes, D. L. Untangling desmosomal knots with electron tomography. Science 302 109-113, 2003. 

X. Electron Tomography Three-Dimensional Electron Microscopy Imaging. 212... 

As shown above, the size and distribution of minute particles are conveniently investigated by high-resolution STEM with a HAADF detector (60,63). The intensity in HAADF images is a monotonic function of the sample thickness and atomic number, a pre-requisite for the electron tomography experiments described below. 

Interestingly, the cellular distribution was analyzed by energy-filtered transmission electron microscopy and electron tomography, demonstrating the presence of free fullerene in the cytoplasm, or associated with nuclear membrane, plasma membrane, lysosomes and, rather surprisingly, with the nucleus (Porter et al., 2006, 2007). 

Porter AE, Muller K, Skepper J, Midgley P, Welland M (2006) Uptake of C60 by human monocyte macrophages, its localization and implications for toxicity Studied by high resolution electron microscopy and electron tomography. Acta Biomaterialia 2 409 119. 

A.J. (2004) Electron tomography a tool for 3D structural probing of heterogeneous catalysts at the nanometer scale. Appl. Catal. A, 260, 71-74. 

Horowitz, R.A., Agard, D.A., Sedat, J.W., and Woodcock, C.L. (1994) The three-dimensional architecture of chromatin in sitw. electron tomography reveals fibers composed of a continuously variable zig-zag nucleosomal ribbon. J. Cell Biol. 125, 1-10. 

The three-dimensional electron tomographical construction of silica-supported metallocene catalysts using conventional TEM (Steinmetz et al 2000), and a novel method for the automated acquisition of tilt series for electron tomography of nanoparticles using STEM have been reported (Zeisse et al 2000). The HAADE-STEM is shown to be capable of determining the compositions of individual nanoparticle catalysts of a few atoms supported on porous substrates (Vaughan et al 1999). 

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