Nucleosides diphosphoric acids

Phosphorylation. Chambers et al. found the reagent useful for the preparation of nucleoside diphosphoric acids (3, R —adenine, uracil, etc.). A nucleotide (I) is converted into the 5 -phosphoramidate (2) by reaction with ammonia and dicyclo-hexylcarbodiimide (DCC), and the amidate (2) is treated with dioxane diphosphate in o-chlorophenol (3 hrs..0 ). The diphosphoric acid (3) is precipitated with petroleum ether and purified as the ammonium salt. Yields are around 90%, whereas when 8.5% phosphoric add was used yields were in the order of 50-60%. However, anhydrous... 

A soln. of 4-chlorophenyl phosphate, triethylamine, and di-a-pyridyl carbonate in abs. acetonitrile kept overnight at 60°, the crude intermediate a-pyridyl phosphate treated with phenyl phosphate in pyridine, and the product isolated as the cyclohexylammonium salt after 6-8 hrs. at room temp. P -phenyl P2-4-chlorophenyl diphosphate. Y 88.4%. F. e. and reactions of -pyridyl phosphates s. W. Kampe, B. 98, 1038 (1965) nucleoside diphosphoric acids s. B. 98, 1045 from a-pyridone instead of di-a-pyridyl carbonate cf. B. 99, 593 (1966). 

T ri-n-hutylamine Nucleoside diphosphoric acids from nucleotides... 

Nucleoside triphosphates are prepared analogously to the diphosphates by the methods a, b, c, and d, representing conversion of a nucleoside phosphoric azolide with inorganic pyrophosphate (Method a), of a nucleoside diphosphoric azolide with inorganic phosphate (Method b), of a phosphoric bisazolide with a nucleoside diphosphate (Method c), and of a diphosphoric bisazolide (bisazolide of pyrophosphoric acid) with a nucleoside phosphate (Method d). 

Diphosphoric acid esters—Nucleoside diphosphates s. 16, 167 labeling of... 

Diphosphoric acid esters from phosphoromorpholidates Nucleoside diphosphates... 

P-Nucleoside P -steroyl diphosphoric acids via diphosphoric acid triesters... 

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