Phosphoric bisazolides

More important than the phosphoric monoazolides are the phosphoric bisazolides in the generation of nucleoside 3 - and 2 -phosphates or 3, 5 -dinucleotides. The phosphoryla-... 

The phosphoric bisazolide reacted only with one nucleoside 3 -OH to give a di-esterified product (cf.[26 32]) however, with a nucleoside 5 -OH component, one or two triazole groups could be substituted, depending on the ratio of the reaction partners.1331 The 3 - or 2 -phosphordiester and -triester were generally obtained in good yield. 

Other phosphoric bisazolides employed for the synthesis of 3 5 -nucleotides are ... 

Nucleoside triphosphates are prepared analogously to the diphosphates by the methods a, b, c, and d, representing conversion of a nucleoside phosphoric azolide with inorganic pyrophosphate (Method a), of a nucleoside diphosphoric azolide with inorganic phosphate (Method b), of a phosphoric bisazolide with a nucleoside diphosphate (Method c), and of a diphosphoric bisazolide (bisazolide of pyrophosphoric acid) with a nucleoside phosphate (Method d). 

Other bisazolides of phosphoric acid include O-phenylphosphoric diimidazolide and -ditriazolide O-phenylphosphoric diimidazolide, for instance, has been prepared from phenylphosphordichloridate and four equivalents of imidazole in benzene. 

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