Nucleic acids restriction endonucleases

The recombinant DNA revolution in biology is rooted in the repertoire of enzymes that act on nucleic acids. Restriction enzymes are a key group among them. These endonucleases recognize specific base sequences in double-helical DNA and cleave both strands of the duplex, forming specific fragments of DNA. These restriction fragments can be separated... 

Heparin-Sepharose Nucleic acid-binding proteins, restriction endonucleases, lipoproteins... 

If recombinant DNA and related techniques in molecular biology are to be applied meaningfully to cestodes, functional nucleic acids have to be prepared such that the DNA can be cleaved by restriction endonuclease enzymes (which digest the double-stranded DNA at highly specific sites, each recognizing a precise nucleotide sequence) and the RNA can be used to... 

The power aind utility of recombinant DNA methods, which include techniques of nucleic acid hybridization, are based on the ability to manipulate DNA in defined ways. These molecular tools are dependent on the commercial availability of a variety of well-characterized enzymes for DNA and RNA modification restriction endonucleases which recognize and cleave specific DNA sequences to create DNA fragments of unique size, DNA and RNA polymerases. 

One-dimensional diffusion can accelerate the formation of site-specific interactions within biopolymers by up to lO -fold (Berg et al., 1981). Such facihtated diffusion is used by transcription factors and restriction endonucleases to locate specific sites on double-stranded DNA (von Hippel and Berg, 1989). The backbone of RNA, like that of DNA, could allow for the facilitated diffusion of proteins. Yet, the facilitated diffusion of a protein along RNA (or any single-stranded nucleic acid) has not been demonstrated previously. 

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