Nucleic Acids and Constituents

Detailed studies on reactions of the [Ru(NH3)5(H20)] cation with nucleic acid and constituent bases have broadened the range of reactions that can take place. The results may be summarized as follows ... 

It is the parent substance of a group of compounds which includes cytosine, thymine and uracil, which are constituents of nucleic acids and barbituric acid and its derivatives, which are important medicinally. 

Reaction of some transition metals with nucleic acids and their constituents. U. Weser, Struct. Bonding (Berlin), 1968, 5, 41-67 (108). 

Weser U (1967) Chemistry and Structure of some Borate Polyol Compounds. 2 160-180 Weser U (1968) Reaction of some Transition Metals with Nucleic Acids and Their Constituents. 5 41-67... 

Reaction of some Transition Metals with Nucleic Acids and their Constituents. Vol. 5, pp. 41-67. 

Metal Ions in Biological Systems. Volume 32. Interactions of Metal Ions with Nucleotides, Nucleic Acids, and their Constituents Ed. Sigel, A. Sigel, H. Marcel Dekker New York, 1996. 

Figure 11.9 Production of rhGH in . coli (as an intracellular protein). Subsequent to fermentation, the cells are collected by centrifugation or filtration. After homogenization, nucleic acids and some membrane constituents are precipitated by the addition of polyethyleneimine. Ammonium sulfate precipitation of the supernatant concentrates the crude rhGH preparation. Chromatographic purification follows, as illustrated...

Nuclei were first isolated by Miescher (1869) from pus cells recovered from discarded surgical bandages. The principle constituent—a phosphorus-rich material then called nuclein—was stained by methyl green. A few years later salmon sperm were shown to contain a phosphorus-rich acidic compound—the nucleic acid—and a basic protein protamine. Further work by Kossel, Levene,... 

The reader is referred to other reviews for detailed discussions of the electronic states and luminescence of nucleic acids and their constituents/0 fluorescence correlation spectroscopy/2) spectroscopy of dye/DNA complexes/0 and ethidium fluorescence assays/4,0 A brief review of early work on DNA dynamics as well as a review of tRNA kinetics and dynamics have also appeared. The diverse and voluminous literature on the use of fluorescence techniques to assay the binding of proteins and antitumor drugs to nucleic acids and on the use of fluorescent DNA/dye complexes in cytometry and cytochemistry lies entirely outside the scope of this chapter. 

The most noteworthy multistage element cycles in which bacteria play important roles are the nitrogen and sulfur redox cycles. The fixation of nitrogen is a reductive process that provides organisms with nitrogen in a form usable for the synthesis of amino acids, nucleic acids, and other cell constituents. In essence, the overall conversion to the key intermediate, ammonia, can be represented as ... 

Cationic dyes. These combine with negatively charged cellular constituents such as nucleic acids and acidic polysaccharides... 

A large number of N-containing compounds of low molecular weight are not precipitated with proteins by 12% trichloroacetic acid. Some small peptides are included in this group. These nonprotein nitrogen (NPN) constituents aggregate about 1 g/liter and account for about 6% of the total N (i.e., 250-350 mg of N per liter). The principal NPN components are listed in Table 1.6 (Wolfschoon-Pombo and Kloster-meyer 1981). The wide variations in concentrations that have been reported for these constituents probably arise from the fact that many of them are metabolites of amino acids and nucleic acids and from the fact that their concentrations in milk depend on the amounts of those substances consumed by the cow. 

Hydroxyl radicals OH, which attack proteins, nucleic acids, and a large variety of other cellular constituents, may also be formed. Although too reactive to diffuse far, they can be generated from H202 by Eq. 18-68. This reaction involves catalysis by Fe ions as shown.5623... 

Prusiner. P. and M. Sundaralingam. 1972. Stereochemistry of nucleic acids and their constituents XXIX. Crystal and molecular structure of allopurinol, a potent inhibitor of xanthine oxid ste Cryst. 

Isildar M, Schuchmann MN, Schulte-Frohlinde D, von Sonntag C (1982) Oxygen uptake in the radiolysis of aqueous solutions of nucleic acids and their constituents. Int J Radiat Biol 41 525-533 Jacobsen F, Flolcman J, Sehested K (1997) Manganese(ll)-superoxide complex in aqueous solution. J Phys Chem A 101 1324-1328... 

Koster R, Asmus K-D (1971) Die Reaktionen chlorierter Athylene mit hydratisierten Elektronen und OH-Radikalen in wassriger Losung. Z Naturforsch 26b 1108—1116 Krishna CM, Decarroz C, Wagner JR, Cadet J, RieszP (1987) Menadione sensitized photooxidation of nucleic acid and protein constituents. An ESR and spin-trapping study. Photochem Photobiol 46 175-182... 

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