Nuclear magnetic resonance spectrometry, protein structure analysis using

Capillary column gas chromatography (GC)/mass spectrometry (MS) has also been used to achieve more difficult separations and to perform the structural analysis of molecules, and laboratory automation technologies, including robotics, have become a powerful trend in both analytical chemistry and small molecule synthesis. On the other hand, liquid chromatography (LC)/MS is more suitable for biomedical applications than GC/MS because of the heat sensitivity exhibited by almost all biomolecules. More recent advances in protein studies have resulted from combining various mass spectrometers with a variety of LC methods, and improvements in the sensitivity of nuclear magnetic resonance spectroscopy (NMR) now allow direct connection of this powerful methodology with LC. Finally, the online purification of biomolecules by LC has been achieved with the development of chip electrophoresis (microfluidics). 

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