Nuclear magnetic resonance saturation transfer experiments

Saturation transfer difference nuclear magnetic resonance experiments have shown that there is a strong interaction between the enzyme and the (R)-enantiomers of the alcohols, but not the (S)-enantiomers (H. W. Anthonsen, unpublished results). As described above, addition of a small amount of pure (R)-alcohols increased the 5-value of kinetic resolution and, moreover, the effect disappeared quickly. The reason is probably that the alcohol moves into the active site where it is esterified. A future goal is to find an additive that can bind irreversibly to this unknown allosteric site, thus causing a lasting effect. 

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