Normetanephrine determination

PH. Gamache, M.L. Kingery and I.N. Acworth, Urinary metanephrine and normetanephrine determined without extraction by using liquid chromatography and coulometric array detection, Clin. Chem., 39, 1825-1830 (1993). 

A sensitive spectrophotometric method based on the strong absorption of the aminochrome-sodium bisulfite addition products (see Section IV, F) at ca. 350 m/x. has been described recently by van Espen128and Oesterling and Tse 277-278 for determining total catecholamines. While not as sensitive as the fluorimetric procedures, this method is considerably more sensitive than the older colorimetric methods based on the visible absorption peak of the aminochromes. Also, it does not have many of the disadvantages (e.g. costly equipment and unstable blanks) often associated with fluorimetric techniques. The basic procedure can be satisfactorily applied to the differential determination of mixtures of adrenaline, noradrenaline, dopamine, metanephrine, and normetanephrine.178... 

Pagliari, R. Cottet-Emard, J.M. Peyrin, L. Determination of free and conjugated normetanephrine and metanephr-ine in human plasma by high-performance hquid chromatography with electrochemical detection. J. Chromatogr. 

Orsulak PJ, Kazuka P, Grab E, Schddkraut JJ. Determination of urinary normetanephrine and metanephrine by radial-compression Uquid chromatography and electrochemical detection. Clin Chem 1983 29 305-9. 

Shoup RE, Kissinger PT. Determination of urinary normetanephrine, metanephrine, and 3-methoxytyramine by liquid chromatography, with amperometric detection. Clin Chem 1977 23 1268-74. 

Taniguchi K, Kakimoto Y, Armstrong M. Quantitative determination of metanephrine and normetanephrine in urine. J Lab Clin Med 1964 64 469-84. 

S. Bninjes, D. Wyhenga and V.J. Johns, Jr, Fluorometric determination of urinary metanephrine and normetanephrine, Clin. Chem., 10, 1-12 (1964). 

J. Jouve, N. Mariotte, C. Sureau and H.P. Muh, High-performance liquid chromatography with electrochemical detection for the simultaneous determination of the methoxy lated amines, normetanephrine, metanephrine and 3-methoxytyramine in urine, J. Chromatogr, llA, 53-62 (1983). 

Willemsen JJ, Ross HA, Wolthers BG, Sweep CGJ, and Kema JP (2001) Evaluation of specific high-performance liquid-chromatographic determinations of urinary meta-nephrine and normetanephrine by comparison with isotope dilution mass spectrometry. Annals of Clinical Biochemistry 38 722-730. 

Determination of Urinary 3-Methoxy-tyramine, Normetanephrine, and Metanephrine in Pheoochrocytoma and Neuroblaistoma by Gas Chromatography... 

Dopamine was produced by incubating liver microsomes of rabbits with p- and m-tyramine, noradrenaline, and normetanephrine by incubation with w-octopamine, and adrenaline by incubation with p- and w-methyloctop-amine/" The injection of labelled tyramine as well as of labelled octopamine in the intact animal (rat) caused the appearance of labelled noradrenaline and normetanephrine in the urine/ As tyramine is also hydroxylated to octopamine, it is possible that the production of noradrenaline from tyramine not only takes place via its conversion to dopamine, but also via its conversion to octopamine. The possibility that octopamine is produced by the hydroxylation of tyrosine to hydroxyphenylserine with ensuing decarboxylation is under discussion. The production of noradrenaline from 3,4-dihydroxyphenylserine, 192-201) amino-acid so far not discovered in the mammal could be demonstrated both in organ extracts and in intact animals. Finally, a transamination of 3-hydroxy- or 3,4-dihydroxyphenylpyruvate to the corresponding amino-acids (m-tyrosin, dopa), and their decarboxylation to m-tyramine and dopamine was observed in intact animals (cats). For the time being it is impossible to determine the importance of the means of formation of catecholamines which have been referred to here. Of the above-mentioned precursor substances, p- and m-tyramine,( octopamine, ... 

A sensitive spectrophotometric method for the determination of urinary noradrenaline, adrenaline, normetanephrine, metanephrine and dopamine involving the formation of aminochrome-sodium bisulphite addition products has been described [197] and is based on an earlier method for noradrenaline and adrenaline [198]. 

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