Norgestrel

Sample preparation 1 mL Serum -I- 3 mL MTBE, vortex for 1 min, centrifuge at 1500 rpm for 5 min. Remove the organic layer and evaporate it to dryness under a stream of nitrogen at room temperature, reconstitute the residue in 100 p,L MeOH, inject an aliquot. 

Guard column 18 mm long (Brownlee) Column 300 X 3.6 10 p,m p.Bondapak C18 Mobile phase MeOH water 80 20 Flow rate 1 Detector UV 254 RIA 

Juzwin, S.J. Tischio, N.S. Flor, S.C. Determination of norgestimate in serum by automated high-performance liquid chromatography and subsequent radioimmunoassay. J.Liq.Chromatogr., 1995, 18, 1851-1861 

Column 250 X 4 10 pm LiChrosorb RP-18 Mobile phase MeOH water 70 30 Flow rate 1 Ipjection volume 25 Detector UV 240 

Simultaneous impurities, norethindrone Interfering ethinyl estradiol 

Chemical Name 13-ethyl-17-hydroxy-1B,19-dinor-17a-pregn-4-en-20-yn-3-one Common Name 17a-ethynyl-1B-homo-19-nortestosterone 

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Figure 11.13 (a-c) Immunoaffinity exti action-SPE-GC-FID ti aces of (a) HPLC-grade water (b) urine (c) urine spiked with /3-19-noitestosti one (0.5 p.g/1) or norethindrone and norgestrel (both 4 p.g/1) (d) SPE-GC-FID ti ace of urine. Reprinted from Analytical Chemistry, 63, A. Faijam et al., Direct inti oduction of large-volume urine samples into an on-line immunoaffinity sample pretreatment-capillary gas cliromatography system, pp. 2481-2487,1991, with permission from the American Chemical Society. 

Di hydro-17a-athynyl-1B-homo-oestradiol 3-methyl ether Norgestrel... 

Contraceptives. Figure 3 Examples of progestins derived from progesterone (pregnanes), 19-nortestosterone (estranes), and norgestrel (gonanes). 

Progestin-only contraceptives (Fig. 4) contain low-doses of progestins (e.g. 350 pg norethindrone or 75 pg norgestrel) that have to be administered daily without interruption. The lowest expected failure rate during the first year of use is 0.5%, while the typical failure rate amounts to 3%. Subdermal implants of norgestrel (216 mg) for sustained release provides for long-term (for up to 5 years) contraceptive effects characterized by failure rates of only 0.05%. Reliable contraception for 3 months can be achieved by an intramuscular injection of a crystalline suspension of 150 mg medroxyprogesterone acetate (Fig. 3) (failure rate 0.3%). 

MonophasicOral Contraceptives 50 meg ethinyl estradiol acetate img norethindrone 50 meg ethinyl estradiol, 1 mg ethynodiol diacetate 50 meg ethinyl estradiol, 0.5 mg norgestrel 35 mg ethinyl estradiol, 1 mg norethindrone 35 meg ethinyl estradiol, 0.5 mg norethindrone 35 meg ethinyl estradiol, 0.4 mg norethindrone 35 meg ethinyl estradiol, 0.25 mg norgestimate 35 meg ethinyl estradiol, 1 mg ethynodiol diacetate... 

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