Non-collagenous protein

Bone protein was extracted following the method described by Sealy (1986). Bone chips were demineralized in a weak HCl solution, then soaked in 0.1 M NaOH to remove base-soluble humic substances. Remaining material, which is mainly collagen, but includes non-collagenous proteins, was... 

Preliminary experiments indicate that several non-collagenous proteins, even with Ca-binding properties, exist in bovine bone217. ... 

The administration of pivaloyl-conjugated beta-lactam antibiotics to healthy volunteers for 54 days reduced mean serum carnitine 10-fold and muscle carnitine, as measured per non-collagen protein, more than 2-fold (62). Long-term treatment of children for 12-37 months to prevent urinary tract infection resulted in serum carnitine concentrations of 0.9-3.6 pmol/l (reference range 23-60 pmol/l). In four cases, muscle carnitine was 0.6-1.4 j,mol/g non-collagen protein (reference range 7.1-19) (63). 

The organic matrix, which constitutes about 32% of the volume of bone, is made up of 90% type 1 collagen, with smaller amounts of non-collagenous proteins such as osteocalcin and osteonectin, which assist in the... 

P5. Picou, D., Halliday, D., and Garrow, J. S., Total body protein, collagen and non-collagen protein in infantile protein malnutrition. Clin. Sei. 30, 345-351... 

The search for a protein-based promoter of hydroxyapatite crystal nucle-ation has been a focus for the isolation and characterisation of the major non-collagenous proteins in bone. Of the proteins characterised to date, BSP II has emerged as the only bona fide candidate for nucleation. BSP is a highly glycosylated and sulfated phosphoprotein found almost exclusively in mineralised connective tissues and has been suggested to constitute approximately 8% of all non-collagenous proteins found in bone. 

---