Nocardia asteroides ATCC

A multi-step screening method was employed to isolate and identify a consortium (identified as CDT-4) formed by Pseudomonas and as Nocardia asteroides (ATCC... 

A peptidolipid fraction was isolated from Nocardia asteroides ATCC 9969. The main component of the mixture, obtained by counter-current distribution, was called peptidolipin NA. After purification, it had m.p. 232-233 "C, [a]o + 42° (chloroform) (106). 

Two strains were isolated and purified, Pseudomonas sp. CDT-4, and Nocardia aster-oides, CDT-4b (ATCC 202160 and 202161, respectively). The microbes were passed through a multiple screen, first to allow growth on dibenzothiophene (DBT) as a sole source of sulfur, and then on fossil fuels, to identify organisms capable of desulfurization without metabolizing the DBT phenyl ring structures. N. asteroides sp. CDT-4b was found to metabolize DBT. The Pseudomonas species was found to utilize trace levels of sulfate from media and was found to be incapable of growth on DBT as a sole source of sulfur. However, the co-culture could remove more than 20% sulfur, with supplementation of a second sulfur-free carbon source. 

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