Proteins NMR structure determination

NMR, protein structure determination, 23, 275 non-enzymatic glycosylation, 14, 261 non-HIV antiviral agents, 36, 119, 38, 213 non-nutritive, sweeteners, V7, 323 non-peptide agonists, 32, 277 non-peptidic d-opinoid agonists, 37,159... 

Kainosho M, Torizawa T, Iwashita Y, Terauchi T, Mei Ono A, Guntert P (2006) Optimal isotope labelling for NMR protein structure determinations. Nature 440 52-57... 

The relative merits of different isotopic enrichment strategies that might be used in solid state NMR protein structure determinations have been discussed by Gehman et aV The basis for comparison of these merits is the determination of the relative uncertainties in rates measured by a generalised dipolar recoupling experiment. The different schemes considered use N and labelling of... 

The secondary structure elements, formed in this way and held together by the hydrophobic core, provide a rigid and stable framework. They exhibit relatively little flexibility with respect to each other, and they are the best-defined parts of protein structures determined by both x-ray and NMR techniques. Functional groups of the protein are attached to this framework, either directly by their side chains or, more frequently, in loop regions that connect sequentially adjacent secondary structure elements. We will now have a closer look at these structural elements. 

A Selection of 3D and 4D NMR Techniques Used in Protein Structure Determination ... 

K. Wuthrich, Six years of protein structure determination by NMR spectroscopy What have we learned in Protein Conformation. Ciba Foundation Symposium 161, John Wiley Sons, Chichester, 1991, pp. 136-149. 

Giintert P, Braun W, Billeter W, Wiithrich K. Automated stereospecific 1H NMR assignments and their impact on the precision of protein structure determinations in solution. J Am Chem Soc 1989 111 3997-4004. 

Sakakibara D, Sasaki A, Ikeya T, Hamatsu J, Hanashima T, Mishima M, Yoshimasu M, Hayashi N, Mikawa T, Walchli M, Smith BO, Shirakawa M, Guntert P, Ito Y (2009) Protein structure determination in living cells by in-cell NMR spectroscopy. Nature 458 102-105... 

Fernandez C, Wuthrich K (2003) NMR solution structure determination of membrane proteins reconstituted in detergent micelles. FEBS Lett 555 144-150... 

Wishart, D. 2005. NMR spectroscopy and protein structure determination applications to drug discovery and development. Current Pharmaceutical Biotechnology 6(2), 105-120. 

K. Wiithrich,/. Biol. Chem. 265, 22059-22062 (1990). Protein Structure Determination in Solution by NMR Spectroscopy. ... 

Protein Data Bank (PDB) Protein structures determined by X-ray and NMR http //www.rcsb.org/pdb/... 

CONTENTS Preface, C. Allen Bush. Thermodynamic Solvent Isotope Effects and Molecular Hydrophobicity, Terrence G. Oas and Eric J. Toone. Membrane Interactions of Hemolytic and Antibacterial Peptides, Karl Lohner and Richard M. Epand. Spin-Labeled Metabolite Analogs as Probes of Enzyme Structure, Chakravarthy Narasimhan and Henry M. Miziorko. Current Perspectives on the Mechanism of Catalysis by the Enzyme Enolase, John M. Brewer and Lukasz Leb-ioda. Protein-DNA Interactions The Papillomavirus E2 Proteins as a Model System, Rashmi S. Hedge. NMR-Based Structure Determination for Unlabeled RNA and DNA, Philip N. Borer, Lucia Pappalardo, Deborah J. Kenwood, and Istvan Pelczer. Evolution of Mononuclear to Binuclear CuA An EPR Study, William E. Antholine. Index. 

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