NMDA electrophysiologic

From electrophysiological studies with in vitro expressed NMDA subunits in cellular systems, it has been concluded that a conventional NMDA receptor must consist of a mixed combination of NR1 splice variants and NR2A-d subunits in order to have full physiological activity (Monyer et al., 1992). This NR1/NR2 expression pattern has also been reported to be a prerequisite for adequate cell surface expression of NMDA receptors (Mcllhinney et al., 1996). Given the tetrameric stoichiometry, any conventional NMDA receptor might consist of two NR1 and two NR2 subunits. 

Priestley, T., Laughton, P., Macaulay, A. J., Hill, R. G., Kemp, J. A. Electrophysiological characterisation of the antagonist properties of two novel NMDA receptor glycine site antagonists, L-695,902 and L-701,324, Neuropharmacology 1996, 35, 1573-1581. 

Sensory transmission from ON axon terminals is mediated by glutamate acting at AMPA and NMDA ionotropic glutamate receptor subtypes (Bardoni et al., 1996 Ennis et al., 1996, 2001 Aroniadou-Anderjaska et al., 1997 Chen and Shepherd, 1997 Keller et al., 1998). The distribution of these receptors in MOB is discussed later (O Section 3.8 and Table 6-3). Electrophysiological studies, described later. 

Electrophysiological experiments have demonstrated that stimulation of the Schaffer collaterals caused an Asp-mediated NMDA response (Fleck et al., 1993). 

Puel JL, Ladrech S, Chabert R, Pujol R, Eybalin M (1991) Electrophysiological evidence for the presence of NMDA receptors in the guinea pig cochlea. Hear Res 57 255-264. 

Alongside these largely electrophysiological studies, others showed that NMDA receptors mediated profound neurotoxicity [26-30] and have a central role in epileptiform discharges [31-32] in vitro and in vivo. As a result, the therapeutic potential of NMDA receptor antagonists has emerged and several agents are under development by pharmaceutical companies [33]. 

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