Nitrogen supplementation

During harvest (and particularly in cool growing regions), it may not be feasible to deal with stuck fermentations immediately. In these cases, the wine should be stabilized against further biological deterioration by draw- 

Where stuck fermentations have been allowed to set over winter, few, if any, viable yeast remain. In these cases, the inoculum must be increased from normally recommended 2-4 x 10 CFU/mL upward to 16-20 x 10 CFU/mL, corresponding to 8-10 lbs/1000 gal (Cone, 1996 personal communication). Additionally, dietary supplements at 2-4 lbs/1000 gal are also recommended. 

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Gmelin Handbook of Inorganic and Organometallic Chemistry, 8th Edition, Nitrogen, Supplement Bl, 280 pp.. Supplement B2, 188 pp., Springer Verlag, Berlin, 1993. 

Jasewicz, L. and Porges, N. 1958. Aeration of whey wastes. I. Nitrogen supplementation and sludge oxidation. Sewage Ind Wastes 30, 555-561. 

Monteiro, F.F. Bisson, L.F. (1992). Nitrogen supplementation of grape juice. 11. Effect on amino-add and urea release following fermentation. Am. J. Enol. Vitic., 43, 11-17. 

The metabolism of branched-chain esters is largely unknown. Based on their structure, it is reasonable to assume that they are formed through esterification of the branched-chain acids formed during amino acid metabolism. It has been reported that nitrogen supplementation of low nitrogen must with ammonium salts has a negative impact on the formation of these compounds during fermentation (Herndndez-Orte et al. 2006 Vilanova et al. 2007). 

Miller, A. C., Wolff, S. R., Bisson, L. F., Ebeler, S. F. (2007) Yeast strain and nitrogen supplementation Dynamics of volatile ester production in Chardonnay juice fermentations. American Journal of Enology and Viticulture, 58,470-483. 

Conversely, there are many low nitrogen forages which do not respond to nitrogen supplementation. Under experimental conditions similar to those of Campling et al. (10) supplemental urea had no effect on the digestion ceilings or intake of wheat straw (0.64% N) or oat straw (0.52% N) fed to sheep and steers, respectively (28). 

The present experiments were undertaken to investigate modified NaOH-treatment procedures and to determine the effect of nitrogen supplementation on both the digestibility and voluntary intake of untreated and treated straw. 

Building the compost pile is called stacking, ricking or "make-up". At this time the pre-wetted starting materials and the nitrogenous supplements are evenly mixed, watered and assembled into a pile. The size, shape and specific physical properties of this pile are very important for optimum composting. These are ... 

After make-up, the mesophiles remain in the cool outer zones while The thermophilic fungi, ac-tlnomycetes and bacteria dominate the inside of the pile. The actinomycetes are clearly visible as whitish flecks forming a distinct ring around the hot center. Bacteria dominate this center area and continue to decompose the nitrogenous supplements, liberating more ammonia. At this point The carbohydrates in the straw are ready for microbial use. 

Proionged ammonification. Oversupplementation with nitrogen. Prolonged time at temp, over 130°F. Reduce nitrogen supplements. Keep temp, under 1 30 ° after pasteurization. Use low temp, ranges during conditioning. 

Free a-amino acid deficiencies in juice are often corrected by the addition of ammonium salts. The most widely used of these, diammonium phosphate or sulfate, are 27% NH4 and 73% P04 or S04 . The maximum level of diammonium phosphate (DAP) permitted by the United States BATF to correct nutritional deficiencies is 968 mg/L, whereas in OIV countries, the maximum allowable addition is 300 mg/L. In Australia, additions are limited by maximum phosphate levels in the wine. In this case, 400 mg/L inorganic phosphate/L is permitted (Henschke and Jiranek, 1993). Historically, urea has been used as a nitrogen supplement during fermentation. However, owing to its demonstrated involvement as a precursor in ethyl carbamate (urethane) formation, the nitrogen source has been eliminated as an approved fermentation adjunct in many countries. 

Although diammonium phosphate is a potent supplement for stimulation of yeast biomass, it is generally not effective as a nitrogen supplement for LAB, which require more complete supplementation in the form of mixtures of amino acids, vitamins, and yeast extract. 

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