Nitrogen-specific chemiluminescence

A nitrogen-specific chemiluminescence detector exists that is very similar to the SCD. The effluent from a column enters a combustion tube with a stainless-steel... 

C12/C14 monoethanolamide, methyl neodecanamide, and JV-methyl C12/C14 glucosa-mides were determined in cleaning formulations by chromatography on Ci) with 80 20 or 75 25 methanol/water, using a nitrogen-specific chemiluminescence detector (156). 

Fujinari EM, Courthaudon LO. Nitrogen-specific liquid-chromatography detector based on chemiluminescence—Application to the analysis of ammonium nitrogen in waste-water. Journal of Chromatography 592, 209-214, 1992. 

EM Fujinari, JD Manes. Nitrogen-specific detection of peptides in liquid chromatography with chemiluminescent nitrogen detector. J Chromatogr A 676 113-120, 1994. 

Nussbaum MA, Baertschi SW, Jansen PJ. Determination of relative UV response factors for HPLC by use of a chemiluminescent nitrogen-specific detector. J Pharm Biomed Anal 2002 27 983-993. 

Fujinary E, Manes JD, Bizanek R, Peptide content determination of crude synthetic peptides by reversed-phase liquid chromatography and nitrogen-specific detection with a chemiluminescent nitrogen detector, J. Chromatogr A, 743 85-89, 1996. 

Nitric oxide release from blood vessels was first detected by chemiluminescence (Palmer et ai, 1987). In the original adaptations of the nitric oxide detector, perfusates from isolated vessels were directly mixed in a reflux chamber containing acetic acid and iodine. The iodine in the reflux chamber served to reduce any nitrites or nitroso-containing groups to nitric oxide, which was stripped from the chamber by a continuous stream of nitrogen or helium that flowed to the chemiluminescent detector. Replacement of the acetic acid with the less volatile trichloroacetic acid reduces problems with contamination of the nitric oxide detector (Dr. D. Harrison, Emory University, Atlanta, Georgia, personal communication, 1991). While extremely sensitive, the use of the acid reflux chamber also reduces the specificity of the assay, raising questions as to whether nitric oxide or a nitrosothiol is EDRF (Myers et ai, 1990). 

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