Nitric oxide fluorescence excitation

Electronic Excitation of NO. Nitric oxide absorbs discretely in the regions 2270, 2150, and 2050 corresponding to the 0,0 1,0 and 2,0 bands of the y system (A2S+ — X tt) and, subsequently, either by fluorescence or coUisional quenching, the ground state is regained. With pressures of 1 to 5 mm. of Hg and a 1600-joule flash the following sequence of reactions produces sufiQcient concentrations of vibrationally excited NO to be observed spectroscopically in absorption (I)... 

The H + NO2 OH + NO reaction provides an excellent example of the use of laser fluorescence detection for the elucidation of the dynamics of a chemical reaction. This reaction is a protot5q)e example of a radical-radical reaction in that the reagents and products are all open-shell free radical species. Both the hydroxyl and nitric oxide products can be conveniently detected by electronic excitation in the UV at wavelengths near 226 and 308 nm, respectively. Atlases of rotational line positions for the lowest electronic band systems of these... 

The use of fluorescent organometallic complexes to label biological substrates is beginning to provide some exciting alternatives to the more traditional organic dyes [126], with suitable iridium [127-129], rhodium [130], platinum [131], rhenium [132-135] and osmium [136] examples having recently been reported. The Re diimine wires (13g and 13h, Fig. 13), have been shown to form complexes with the nitric oxide synthase mutant 5114 [137]. Steady-state luminescence measurements with 13h establish a dissociation constant of 100 nM, while 13h binds with a... 

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