Nitella flexilis

Such a study has been performed on a model plant system, the Nitella flexilis cell wall [1, 2, 3]. This freshwater alga has giant intemodal cells whose easily isolated cell walls constitute a simplified model of higher plant cell walls it has no lignin and its pectin is not methylesterified. Isolated cell walls are cut in pieces and distributed in different lots over the whole exchange isotherm to reduce variability between experimental points. 

Fig. 3. EPR spectra of Nitella flexilis cell walls with cupric ions on 9% (a) or 100% (b) of the uronates. The spectra have been recorded at two temperatures (arbitrary intensities). The sharp small peaks indicate g = 2.0028. ...

Van Cutsem P and Gillet C (1982) Activity coefficient and selectivity values of Cu, Zn and Ca ions adsorbed in the Nitella flexilis L. cell wall during triangular ion exchanges J Exp Bot 33 847-853. 

Haematococcus pluvialis Nitella flexilis Scenedesmus obliquus Spirogyra majuscula S. varians Ulva lactuca Valonia utricularis Phaeophyta (brown) Ascophyllum nodosum Chorda tomentosa Cutleria multifida... 

The spear-type glass microelectrode (Hinke-type) (2) (Figure la) has been used to determine the intracellular pH of the crab muscle fibers (3,4), cytoplasm of the giant squid axon (5), rat sartorius muscle fibers (6), rat atrial muscle fibers (7), vacuole of the algae Nitella flexilis (8), rat kidney tubular fluid (9,10), nerve cells of mollusk ganglia (11), and skeletal muscle fibers of the rat (4,12,13,14). 

Hirakawa, S., Yoshimura, H., Measurements of the Intracellular pH in a Single Cell of Nitella flexilis by Means of Micro-Glass pH Electrodes, Jap. J. Physiol., 45-55 (1964). 

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