Nicotinamide adenine nucleotide

The fact that adenosine and its derivatives are azo coupling components is used for immobilizing nicotinamide-adenine nucleotide (NAD+) for affinity chromatography purposes. In 12.58 NAD+ is bonded to a matrix through an azo bond. Compound 12.58 is used for the purification of dehydrogenase enzymes (Hocking and Harris, 1973). 

Naphthoquinone diazides 32, 284ff., see also Quinone diazides 2,3-Naphthotriazole, formation 132 f. Negations, psycholinguistics of 215 Nesmeyanov reactions 273 ff. Nicotinamide-adenine nucleotide (NAD+) 328 f. 

Pyruvate is reduced to 1-lactate by reduced nicotinamide-adenine-nucleotide (NADH) in the presence of lactate-deshydrogenase ... 

Reduction of NADP by NADH is catalysed by H -transhydrogenase, a ApH consumer competent in the H transfer between two nicotinamide adenine nucleotide in a ApH+-linked fashion. As a source of ApH+, respiration or ATP hydrolysis can be used [5],... 

Figure 2a. Nicotinamide—adenine nucleotide cofactors (oxidised).

Figure 16.2 Absorption spectra of the oxidized and reduced forms of the nicotinamide adenine nucleotides (NAD and NADP)...

Sanwal,B.D. Regulatory mechanisms involving nicotinamide adenine nucleotides as allosteric effectors. I. Control characteristics of malate dehydrogenase. J. Biol. Chem. 244, 1831-1837(1969)... 

Arene dioxygenases catalyze the first step in the metabolism of unactivated aromatic compounds, yielding cw-dihydrodiol of aromatics. As shown in eq. (22), the reaction requires nicotinamide adenine nucleotide (NADH) and molecular oxygen. Substrate specificity of enzymes is high, and products shown in Fig. 27 are produced by benzene-[351, 352], toluene- [353-356], naphthalene- [357-361], biphenyl- [362-364], benzoate-[365-370], and phthalate-dioxygenases [371-378]. In the cases of benzoate [379], 4-sulphobenzoate [380], and 6>-nitrotoluene [381], catechols are formed via unstable dihydroxylated intermediates as shown in eq. (23)... 

If the Chibcron blue F3GA (11) [37] or Prussian red HE3B (12) are bonded to a gel for support, the proteins or enzymes that interact with the nicotinamide adenine nucleotide can be selectively adsorbed and separated because the structure of these dyes is similar to that of the nicotinamide adenine nucleotide [38]. There are also examples of selective separation using a dye that exhibits photochromism by polarity changes of the molecule. 

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