NEWPORT GREEN DCF

CA Index Name Spiro[isobenzofuran-l(3//),9 -[9//] xanthene]-5-carboxamide, N-[4-[bis(2-pjnidinylmethyl) amino]phenyl]-2, 7 -dichloro-3, 6 -dihydroxy-3-oxo-, potassium salt (1 2) 

Merck Index Nnmber Not listed Chemical/Dye Class Xanthene Molecular Formula C39H24CI2K2N4O6 Molecular Weight 793.74 Physical Form Solid Solubility Soluble in water Melting Point 200 °C Absorption (Xmax) 506 nm Emission (Xmax) 535 nm Synthesis Synthetic method  

Staining Applications Zinc ions chromium manganese iron cobalt copper nickel cadmium  

Biological Applications Zinc indicator early diagnosis of prostate cancer treating herpes virus infection  

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Recently a lifetime-probe (Newport Green DCF) for imaging Zn + has been described [124], and also reports have appeared of non-ratiometric lifetime probes for quantifying Na+ [ 125] and for Mg (magnesium-green, Mag-quin-2, Mag-quin-1), Cl (MQAE,SPQ), heavy metals (bis-BTC) andK+ (PBFI) [126]. 

Fig. 2 Osteoclasts were cultured for 2 weeks on titanium foil before stained with Newport Green DCF and documented with confocal fluores-cence microscopy [14]. Note the green stained granular areas in the cyto-plasm as well as in the nucleus representing titanium distribution in the bi-nucleated cell (blue nuclear stain)...

Cadosch D, Meagher J, Gautschi OP et al. (2009) Uptake and intracellular distribution of various metal ions in human monocyte-derived dendritic cells detected Newport Green DCF diacetate ester. J Neuro-sci Meth 178 182-187. 

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