Neuronal cytoplasmic protein

Myelin associated glycoprotein neuronal cytoplasmic protein 3 (MAG/Ncp 3)... 

MAG is one of a set of cell-surface glycoproteins expressed by myelin-forming cells which have been hypothesized to mediate the apposition of the myelin sheath to the axon. Two forms of MAG are known which, similarly to NCAM, differ in the intracytoplasmic domain as a result of alternative splicing. The carboxy-terminal 318 amino acids of MAG are identical to the partial sequence so far obtained of a protein that has been designated as rat neuronal cytoplasmic protein 3 [184], However, the latter was previously believed to be a neuropeptide precursor and to be expressed in specific groups of neurons rather than in glial cells. The reason for this discrepancy is unclear. 

The appropriateness of using platelets, as a cell mirroring some neurochemical processes, finds its rationale in the numerous similar features of platelets and neurones Platelets store and release neurotransmitters and express appropriate neurotransmitter transporters and some neurone-related proteins such as NMDA receptors. Along these lines, different authors reported abnormalities in platelets physiology and function in AD. At first, Zubenko and colleagues described an alteration in platelet membrane fluidity in AD patients [99]. Other studies, performed on platelets obtained from patients with moderate to severe AD, reported cytoskeletal abnormalities, cytochrome oxidase deficiency, abnormal cytoplasmic calcium... 

Selden LA, Kinosian HJ, Estes JE, Gershman LC (1999) Impact of profilin on actin-bound nucleotide exchange and actin polymerization dynamics. Biochemistry 38 2769-2778 Sharma A, Lambrechts A, Hao le T, Le TT, Sewry CA, et al (2005) A role for complexes of survival of motor neurons (SMN) protein with gemins and profilin in neurite-Uke cytoplasmic extensions of cultured nerve cells. Exp Cell Res 309 185-197... 

DiFiglia, M., E. Sapp, K. Chase et al. Huntingtin is a cytoplasmic protein associated with vesicles in human and rat brain neurons. Neuron 14(5), 1995 1075-81. 

All of the known neurotransmitters synthesised in mammalian neurons share certain chemical properties all are low-molecular-weight, water-soluble compounds which are ionised at the pH of body fluids. All are synthesised primarily in the parts of the neuron (the synaptic bulbs, or nerve terminals) which form synaptic contacts with the cells to which the neuron transmits signals (the post-synaptic cells), and all are stored within subcellular organelles (synaptic vesicles) or are attached to cytoplasmic proteins, prior to being released into a synapse (Fig. 1). Release occurs when the neuron that synthesised the neurotransmitter is depolarised. Thereafter, some of... 

Grunewald A, Lax NZ, Rocha MC et al. (2014) Quantitative quadruple-label immunofluorescence of mitochondrial and cytoplasmic proteins in single neurons from human midbrain tissue. J Neurosci Methods. http //dx.doi.Org/10.1016/j.jneumeth. 2014.05.026... 

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